ATM蛋白激酶-检测点激酶2通路在氟抑制大鼠曲细精管上皮支持细胞增殖中的作用

发布时间：2018-03-26 18:12

本文选题：氟　切入点：曲细精管上皮支持细胞　出处：《环境与健康杂志》2015年01期

【摘要】：目的观察ATM蛋白激酶-检测点激酶2(ATM-Chk2)通路在氟抑制大鼠曲细精管上皮支持细胞增殖中的作用。方法将18日龄SPF级雄性SD大鼠曲细精管上皮支持细胞分别暴露于含0(对照)、0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠的血清培养基。分别于染毒24、48、72 h,采用MTT法测定支持细胞的增殖情况;于染毒24 h,采用实时荧光定量PCR技术检测支持细胞内ATM、Chk2 m RNA的表达水平。结果与对照组比较,染毒24、48、72 h时,4.00 mmol/L氟化钠染毒组大鼠曲细精管上皮支持细胞的抑制率均较高,差异有统计学意义(P0.05);且随着氟化钠染毒剂量的升高和染毒时间的延长,氟对曲细精管上皮支持细胞的抑制率均呈上升趋势。与对照组比较,1、2 mmol/L氟化钠染毒组曲细精管上皮支持细胞ATM和Chk2 m RNA的表达水平均升高,而4 mmol/L氟化钠染毒组ATM和Chk2 m RNA的表达水平均降低,差异有统计学意义(P0.05);且随着氟化钠染毒剂量的升高,曲细精管上皮支持细胞ATM、Chk2 m RNA的表达水平均呈下降趋势。结论不同剂量氟抑制生殖细胞增殖可能是通过干扰曲细精管上皮支持细胞中DNA损伤检验点ATM、Chk2的表达而实现的。
[Abstract]:Objective to observe the role of ATM protein kinase-detection point kinase 2 ATM-Chk2 pathway in inhibiting the proliferation of rat seminiferous tubule epithelial Sertoli cells (Sertoli cells) in rats. Methods 18 days old male SD rats with SPF grade were exposed to seminiferous tubule epithelial Sertoli cells containing fluorine. The serum culture medium of 0.25 ~ 0.50 ~ 1.00 ~ 2.00 ~ 4.00 mmol/L sodium fluoride was used to determine the proliferation of Sertoli cells by MTT method. The expression of Chk2 m RNA in Sertoli cells was detected by real-time fluorescence quantitative PCR at 24 h after exposure. Results compared with the control group, the inhibitory rates of Sertoli cells of the rats exposed to sodium fluoride for 4. 00 mmol/L at 72 h were higher than those in the control group. The difference was statistically significant (P 0.05), and with the increase of the dose of sodium fluoride and the prolongation of the time of exposure, The inhibitory rates of fluoride on Sertoli cells of seminiferous tubules showed an increasing trend. Compared with the control group, the expression of ATM and Chk2 m RNA in seminal tubule epithelial Sertoli cells exposed to sodium fluoride for 1 mmol/L was higher than that in the control group. The expression levels of ATM and Chk2 m RNA in 4 mmol/L sodium fluoride group were significantly lower than those in the control group (P 0.05), and with the increase of sodium fluoride dose, The expression of ATMN Chk2 m RNA in seminiferous tubule epithelial Sertoli cells showed a decreasing trend. Conclusion different doses of fluoride inhibit the proliferation of germ cells by interfering with the expression of ATMnChk2 in the DNA damage detection site of seminiferous tubule epithelial Sertoli cells.
【作者单位】：广东药学院公共卫生学院;广东省分子流行病学重点实验室;
【基金】：2013年地方高校国家级大学生创新训练计划项目(201310573008)
【分类号】：R114

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