对虾中T-2毒素的残留规律及其对雄性小鼠的遗传毒性效应

发布时间：2018-03-29 02:20

本文选题：对虾　切入点：T-毒素　出处：《现代食品科技》2015年02期

【摘要】：研究了对虾中T-2毒素急性暴露的残留规律,并探明其对雄性小鼠的遗传毒性效应。实验以LC-MS/MS检测肌肉注射染毒后,对虾中T-2毒素残留量,并经灌胃,检测毒虾中T-2毒素对小鼠遗传毒性的危害。结果发现,对虾中游离态T-2毒素的残留量与暴露剂量呈正相关,表现蓄积性,并且不同组织的蓄积量差异较大,以肌肉中含量最低,接近于零。经毒虾灌胃后,小鼠的精子畸形率及微核率也随暴露剂量逐渐增大。与对照组相比,高剂量灌胃组的小鼠精子畸形率和微核率出现显著性变化(P0.05),高剂量组的四个对虾组织都具有毒性,其中以对虾肌肉和肝胰腺的毒性最强,其次为肠道。表明T-2毒素在对虾体内具有隐蔽性,特别是在肌肉中,游离态T-2毒素易于被转化为隐蔽态T-2毒素,从逃避检测,并产生二次危害隐患。
[Abstract]:The residue rule of acute exposure to T-2 toxin in prawn was studied, and the genotoxic effect of T-2 toxin on male mice was investigated. The residual T-2 toxin in prawn was detected by LC-MS/MS after intramuscular injection. The results showed that the residues of free T-2 toxin in shrimp were positively correlated with the exposure dose, and showed accumulative properties, and there were great differences in the accumulation of T-2 toxin in different tissues, the lowest in muscle, the results showed that T-2 toxin in prawn was harmful to the genetic toxicity of mice, and the content of free T-2 toxin in shrimp was positively correlated with the exposure dose. The sperm deformity rate and micronucleus rate of mice increased with the exposure dose. The sperm deformity rate and micronucleus rate of mice in the high dose group showed significant changes (P 0.05). The four shrimp tissues in the high dose group were all toxic, especially in the shrimp muscle and hepatopancreas. Secondly, intestinal tract showed that T-2 toxin was concealed in shrimp, especially in muscle. Free T-2 toxin was easily transformed into hidden T-2 toxin, which could be detected from escape, and had secondary hazard hidden danger.
【作者单位】：广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院;广西壮族自治区产品质量监督检验研究院;
【基金】：国家自然科学基金资助项目(31171634,31371777)
【分类号】：TS254.7;R114

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