竹荪醇提物对脂多糖诱导的RAW264.7细胞炎症反应的影响

发布时间：2018-03-30 16:38

本文选题：竹荪醇提物　切入点：RAW.细胞　出处：《营养学报》2017年04期

【摘要】：目的探讨竹荪醇提物对LPS诱导的RAW264.7细胞肿瘤坏死因子(TNF-α)、一氧化氮(NO)的分泌,TNF-α、诱导型一氧化氮合酶(iNOS)、白细胞介素6(IL-6)、白细胞介素10(IL-10)的m RNA水平以及核转录因子(NF-κB)p65蛋白表达的影响及可能的抗炎机制。方法以不同浓度(100、200、400μg/ml)的竹荪醇提物作用于LPS(1μg/ml)诱导的RAW264.7小鼠巨噬细胞,采用ELISA法和Griess法检测炎性介质TNF-α和NO的分泌量;RT-PCR法测定促炎介质TNF-α、iNOS、IL-6和抗炎介质IL-10的m RNA水平;Western blot检测NF-κB p65蛋白的表达水平。结果与LPS组相比,200、400μg/ml的竹荪醇提物能显著抑制LPS诱导的RAW264.7细胞TNF-α和NO的释放(P0.01);且不同程度抑制RAW264.7细胞中促炎介质iNOS、TNF-α和IL-6的m RNA表达水平(P0.01),剂量依赖性的促进抗炎介质IL-10的m RNA表达水平(P0.01);不同浓度的竹荪醇提物均可显著抑制LPS诱导RAW264.7细胞中NF-κB p65蛋白的表达,差异有统计学意义(P0.01)。结论竹荪醇提物可抑制LPS诱导的RAW264.7细胞TNF-α和NO的释放,其抗炎作用的发挥可能与抑制NF-κB p65蛋白的表达,从而调节炎性介质的基因水平有关。
[Abstract]:Objective to investigate the level of m RNA and the transcription factor NF- 魏 B)p65 protein of LPS induced tumor necrosis factor TNF- 伪 (no), inducible nitric oxide synthase (iNOS), interleukin 6 (IL 6) and interleukin 10 (IL 10) in RAW264.7 cells induced by alcohol extract of bamboo sun. Methods LPS(1 渭 g / ml (100 渭 g / ml) was used to induce macrophages in RAW264.7 mice. ELISA and Griess were used to detect the secretion of TNF- 伪 and no. RT-PCR was used to detect the levels of IL-6 and m RNA of TNF- 伪 and IL-10. The expression of NF- 魏 B p65 protein was detected by Western blot. To inhibit the release of TNF- 伪 and no in RAW264.7 cells induced by LPS, and to inhibit the expression level of m RNA of iNOS TNF- 伪 and IL-6 in RAW264.7 cells to varying degrees, and to promote the expression level of m RNA of IL-10 in a dose-dependent manner, and to increase the expression level of m RNA of IL-10 in a dose-dependent manner. The alcohol extract could significantly inhibit the expression of NF- 魏 B p65 protein in RAW264.7 cells induced by LPS. Conclusion the alcohol extract can inhibit the release of TNF- 伪 and no in RAW264.7 cells induced by LPS, and its anti-inflammatory effect may be related to the inhibition of the expression of NF- 魏 B p65 protein and the regulation of the gene level of inflammatory mediators.
【作者单位】：辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室;
【基金】：国家级大学生创新创业训练计划(No.201610165042) 辽宁省教育厅科学研究一般项目(No.L201683675)
【分类号】：R151

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