苯并芘和4-溴联苯醚联合暴露对大鼠卵巢功能的影响及机制探讨

发布时间：2018-04-01 05:27

本文选题：苯并芘　切入点：-溴联苯醚　出处：《山东医药》2017年18期

【摘要】：目的观察苯并芘和4-溴联苯醚(BDE-3)单独及联合暴露对雌性大鼠卵巢功能的影响,并探讨其可能的损伤机制。方法将60只大鼠随机分为4组,于动情期A、B、C、组分别给予溶于玉米油的苯并芘、BDE-3、苯并芘联合BDE-3 5 mg/(kg·d)等容灌胃法染毒暴露,D组给予玉米油5 mg/(kg·d),连续灌胃28 d。于动情间期眼球取血,脱椎处死并获取卵巢组织。采用ELISA法检测血清孕酮,分别采用荧光定量PCR、Western blot法检测卵巢组织中的芳香烃受体(AhR)mRNA、细胞色素P450家族成员1A1(CYP1A1)蛋白。结果 A、B、C组血清孕酮水平及卵巢组织AhR mRNA、CYP1A1蛋白相对表达量均较D组增高,且C组各指标均高于A、B组(P均0.05);A、B组比较,差异无统计学意义。结论苯并芘和BDE-3暴露对卵巢功能产生损伤效应,且联合暴露可产生协同作用;其损伤机制可能是通过AhR调控CYP1A1表达,从而增强对卵巢组织的毒性效应。
[Abstract]:Objective to observe the effects of benzopyrene and 4-brominated diphenyl ether (BDE-3) exposure alone or in combination on ovarian function in female rats and to explore the possible mechanism of its damage. Methods 60 rats were randomly divided into 4 groups. During estrous period, group D was exposed to benzopyrene BDE-3 dissolved in corn oil, benzopyrene combined with BDE-3 5 mg/(kg d. Group D was treated with corn oil for 5 mg/(kg d, and continuous gavage for 28 days. Blood was taken from eyeball during estrus period. The serum progesterone was detected by ELISA method. Fluorescence quantitative PCR blot was used to detect the aromatic receptor AhR1mRNAs and cytochrome P450 family members of CYP1A1.Results the serum progesterone level and the relative expression of AhR mRNA-CYP1A1 protein in ovarian tissue were higher than those in D group, and the level of progesterone in serum and the relative expression of AhR mRNA-CYP1A1 protein in ovarian tissue were higher in group A than in group D. All indexes in group C were higher than those in group A (P = 0.05). There was no significant difference between group A (P) and group A (P = 0.05). Conclusion Benzo pyrene and BDE-3 exposure can damage ovarian function, and combined exposure can produce synergistic effect. The mechanism of its damage may be that the expression of CYP1A1 is regulated by AhR, thereby enhancing the toxic effect on ovarian tissue.
【作者单位】：桂林医学院;
【基金】：广西自然科学基金资助项目(2013GXNSFBA019190) 广西高校科学技术研究项目(2013ZD044)
【分类号】：R114

【相似文献】