砷冶炼厂工人微小RNA548和三价砷甲基转移酶mRNA表达水平与砷代谢产物的关系

发布时间：2018-04-02 13:11

本文选题：砷　切入点：代谢产物　出处：《环境与职业医学》2015年09期

【摘要】：[目的]探讨微小RNA548(mir548)和三价砷甲基转移酶(AS3MT)m RNA表达及其与砷甲基化代谢产物的关系。[方法]2011年9月采用整群抽样的方法选择云南某2个砷冶炼厂78名工人作为接触组,选择该地非接触砷作业居民23人为对照组。使用带砷预处理系统的原子吸收分光光度法检测两组人群尿中的无机砷(i As)、甲基砷酸(MMA)和二甲基砷酸(DMA)含量,并计算相应的百分含量和一、二级甲基化指数;通过基因分析软件分析影响AS3MT的主要m RNA。实时荧光定量PCR检测研究对象外周血mir548和AS3MT m RNA表达。采用Spearman方法对各项指标进行相关分析。[结果]接触组尿中i As、MMA、DMA、一级甲基化指数和MMA%的中位数为134.54、235.88、563.57μg/g肌酐、1.61及19.01%,对照组为2.00、1.35、14.28μg/g肌酐、1.04及6.45%,接触组均高于对照组(P0.05)。二级甲基化指数和DMA%则为接触组低于对照组(P0.05),分别为3.41、61.98%和12.31、78.01%。接触组外周血mir548表达低于对照组(5.08±1.11与6.49±1.68),AS3MT m RNA表达水平高于对照组(10.71±2.52与9.19±2.11),均P0.01。相关分析发现:外周血淋巴细胞mir548与AS3MT m RNA表达及i As、MMA、DMA及MMA%呈负相关关系(P0.01);外周血AS3MT m RNA表达与尿中i As、MMA、DMA、MMA%及外周血mir548表达与DMA%均呈正相关关系(P0.01)。[结论]mir548与AS3MT m RNA表达有负相关,两者与砷不同甲基化代谢产物浓度和百分含量存在关联。
[Abstract]:[objective] to investigate the expression of microRNA548mm548) and trivalent arsenic methyltransferase AS3MTM RNA and their relationship with arsenic methylation metabolites. [methods] in September 2011, 78 workers from two arsenic smelters in Yunnan were selected as exposure group by cluster sampling. In this study, 23 non-exposed arsenic workers were selected as control group. The contents of inorganic arsenic (as), methyl arsenate (MMA) and dimethyl arsenate (DMA) in urine were determined by atomic absorption spectrophotometry (AAS) with arsenic pretreatment system in the two groups. The corresponding percentage content and 1, 2 methylation index were calculated. The expression of mir548 and AS3MT m RNA in peripheral blood of the subjects studied by real-time fluorescence quantitative PCR were analyzed by gene analysis software. The correlation analysis of each index was carried out by Spearman method. [results] in the exposed group, the expression of mir548 and AS3MT m RNA in urine was determined by real time fluorescence quantitative PCR. The median of grade I methylation index and MMA% was 134.54235.88H563.57 渭 g / g creatinine 1.61 and 19.01 渭 g / g, and that of the control group was 2.000.35 ~ 14.28 渭 g / g / g creatinine 1.04 and 6.45% respectively. The exposure group was higher than the control group (P 0.05), and the second stage methylation index and DMA% were lower than those of the control group (3.41%, 61.98% and 12.31%, 78.01%, respectively). The expression of mir548 in blood was lower than that in control group (5.08 卤1.11 and 6.49 卤1.68). The expression of AS3MT m RNA was significantly higher than that in control group (10.71 卤2.52 and 9.19 卤2.11). The correlation analysis showed that the expression of mir548 in peripheral blood lymphocytes was negatively correlated with the expression of AS3MT m RNA, and the expression of AS3MT m RNA in peripheral blood was negatively correlated with the expression of AS3MT m RNA and MMA%, while the expression of AS3MT m RNA in peripheral blood was negatively correlated with the expression of AS3MT m RNA and MMA%. There was a positive correlation between the expression of MMA% in urine and the expression of mir548 in peripheral blood. [conclusion] there was a negative correlation between the expression of mir548 and AS3MT m RNA. They were correlated with the concentration and percentage of arsenic methylation metabolites.
【作者单位】：云南省疾病预防控制中心职业卫生与中毒控制所;
【基金】：国家自然科学基金(编号:81160343;30860238) 云南省应用基础研究基金(编号:2011FB147) 云南省高层次卫生技术人才培养专项经费(编号:D-201250)
【分类号】：R131

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