头发中尼古丁和可替宁的超高效液相色谱-串联质谱测定法

发布时间：2018-04-02 13:23

本文选题：头发　切入点：尼古丁　出处：《环境与健康杂志》2017年07期

【摘要】：目的建立头发中尼古丁和可替宁的超高效液相色谱串联质谱(UPLC-MS-MS)测定方法。方法头发样品用Na OH溶液完全消解后用二氯甲烷+甲醇(3+1)萃取,以10 mmol/L乙酸铵(含0.1%甲酸)-乙腈作为流动相进行梯度洗脱,超高效液相色谱串联质谱多反应监测(MRM)模式测定,内标法定量。结果尼古丁在2~200μg/L的范围内,所得回归方程为y=1.296 7x-2.379 9,r=0.999 6;可替宁在0.2~20μg/L的范围内,所得回归方程为y=1.099 2x-0.005 15,r=0.999 9。以3倍信噪比计算,当取样量为50 mg时,尼古丁和可替宁检出限分别为0.01μg/g和0.001μg/g。该方法所得尼古丁的平均回收率在75.0%~97.0%之间,可替宁的平均回收率在90.0%~102.0%之间,RSD5%。结论本方法简便、快速、准确,适用于被动吸烟人群头发中尼古丁和可替宁含量的测定。
[Abstract]:Objective to establish a method for the determination of nicotine and cotinine in hair by UPLC-MS-MS.Methods hair samples were completely digested with NaOH solution and extracted with methanol-31 dichloromethane. 10 mmol/L ammonium acetate (containing 0.1% formate-acetonitrile) was used as mobile phase for gradient elution, and the multireaction monitoring method was used to determine the amount of nicotine in the range of 2 渭 g / L ~ (2 渭 g / L). The regression equation is yang1.296 7x-2.379 / r 0.999 6, and the regression equation is yang1.099 2x-0.005 15r0.999 9 in the range of 0.2 渭 g / L for ketenin, calculated at 3 times signal-to-noise ratio (SNR), when the sample size is 50 mg, The detection limits of nicotine and cotinine were 0. 01 渭 g / g and 0.001 渭 g / g respectively. The average recoveries of nicotine and cotinine were 75.0% and 102.0%, respectively. Conclusion this method is simple, rapid and accurate. It is suitable for the determination of nicotine and cotinine in hair of passive smokers.
【作者单位】：天津市疾病预防控制中心卫生检测所理化室;
【分类号】：R163

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