亚慢性铅镉联合暴露对大鼠肝功能的影响及α-硫辛酸的保护作用

发布时间：2018-04-02 17:59

本文选题：亚慢性　切入点：铅　出处：《黑龙江畜牧兽医》2017年03期

【摘要】：为了研究铅镉联合暴露对大鼠肝功能的影响以及α-硫辛酸的保护作用,试验以300 mg/L铅(Pb)、50 mg/L镉(Cd)单独或联合(Pb+Cd)采用自由饮水方式染毒SD大鼠,α-硫辛酸保护组在Pb、Cd自由饮水的同时按体重以50 mg/kgα-硫辛酸灌胃,12周后测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清碱性磷酸酶(ALP)活性及总蛋白(TP)、白蛋白(ALB)的含量。结果表明:与对照组相比,铅组、镉组ALT活性极显著升高(P0.01),AST和ALP活性极显著下降(P0.01),镉组TP和ALB含量显著或极显著下降(P0.05或P0.01),铅组TP和ALB含量无显著性差异,铅镉联合组ALT和AST活性极显著升高(P0.01),ALP活性、TP和ALB含量无显著性差异;与铅、镉单独染毒组相比,铅镉联合组ALT、AST活性极显著升高(P0.01),ALP活性、TP和ALB含量无显著性差异;与相应染毒组相比,α-硫辛酸保护组大鼠肝功能各项指标均有所恢复,部分具有统计学意义(P0.05)。说明铅、镉单独或联合暴露对大鼠肝功能有损伤作用,α-硫辛酸可在一定程度上降低铅、镉单独或联合引起的肝功能损伤。
[Abstract]:In order to study the effects of lead and cadmium combined exposure on liver function and the protective effect of 伪 -lipoic acid in rats,The activity of alt, aspartate aminotransferase (AST), serum alkaline phosphatase (ALP) and the contents of total protein (TPN) and albumin (ALB) were studied.The results showed that compared with the control group, the activity of ALT in cadmium group was significantly higher than that in control group. The activities of P0.01T and ALP in cadmium group were significantly decreased. The contents of TP and ALB in cadmium group were significantly decreased (P0.05 or P0.01), but the contents of TP and ALB in lead group were not significantly different.Compared with the corresponding exposure group, the liver function indexes of 伪 -lipoic acid protective group were all recovered, and some of them had statistical significance (P 0.05).It was suggested that lead and cadmium exposure alone or in combination could damage liver function in rats, and 伪 -lipoic acid could reduce the liver function damage caused by lead and cadmium alone or in combination to some extent.
【作者单位】：扬州大学兽医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;扬州市邗江区畜牧兽医站;
【基金】：国家自然科学基金项目(31302058) 中国博士后科学基金项目(2015M581874) 江苏省博士后科研资助计划项目(1501072A)
【分类号】：R114

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