2010-2013年湖南省市售水产品中副溶血性弧菌污染状况及病原特征分析

发布时间：2018-04-09 15:54

本文选题：副溶血性弧菌　切入点：水产品　出处：《实用预防医学》2016年12期

【摘要】：目的了解湖南省市售水产品中副溶血性弧菌污染状况和病原特征。方法 2010-2013年在湖南省14个市的集贸市场和超市,按照无菌采样原则采集动物性水产品1 263份,分离副溶血性弧菌,进行毒力基因、耐药表型和脉冲场凝胶电泳(PFGE)分子分型。结果 1 263份水产品中,221份样品检出副溶血性弧菌,检出率为17.50%。3株副溶血性弧菌检出trh基因,1株检出tdh基因,毒力基因携带率为1.81%。分离菌株对氨苄青霉素耐药率最高,达30.32%。其次为对氯霉素(1.81%)、复方新诺明(1.36%)和四环素(0.45%),未发现多重耐药菌株。分离菌株经NotⅠ酶切后,脉冲场凝胶电泳带型相对分散。结论 2010-2013年湖南省市售水产品中副溶血性弧菌污染率高,部分菌株携带毒力基因,并具有一定的耐药性。
[Abstract]:Objective to understand the Hunan commercial parahemolytic vibrio in aquatic products and contamination characteristics. Methods 2010-2013 years in 14 cities of Hunan Province, bazaars and supermarkets, in accordance with the principle of aseptic sampling acquisition aquatic products of animal 1263, separation of Vibrio parahaemolyticus, virulence gene, resistance phenotype and pulsed field gel electrophoresis (PFGE) molecular typing. Results 1263 of 221 samples of aquatic products, the detection of Vibrio parahaemolyticus, the detection rate of 17.50%.3 strains of Vibrio parahaemolyticus TRH gene, 1 strains of TDH gene, virulence gene carrying rate of 1.81%. isolates were resistant to ampicillin was the highest, followed by up to 30.32%. of chloramphenicol (1.81%), SMZ-TMP (1.36%) and tetracycline (0.45%), was not found. Multidrug resistant strains of isolates by Not I digestion, pulsed field gel electrophoresis with relative dispersion. Conclusion 2010-2013 years sold in Hunan Province in aquatic products The contamination rate of Vibrio parahaemolyticus is high. Some strains carry the virulence gene and have certain resistance.

【作者单位】：湖南省疾病预防控制中心;
【基金】：湖南省卫生厅科研基金资助项目(B2014-132)
【分类号】：R155.55

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