黑木耳多糖对大鼠心肌细胞过氧化损伤的保护作用

发布时间：2018-04-12 00:37

本文选题：黑木耳多糖 + 氧化损伤　；参考：《时珍国医国药》2015年02期

【摘要】：目的研究黑木耳多糖对H2O2致大鼠心肌细胞氧化损伤的保护作用及其机制。方法用H2O2诱导心肌细胞H9c2建立氧化应激模型,用低、中、高质量浓度的黑木耳多糖进行干预。采用MTT比色法检测心肌细胞的存活率,用试剂盒检测细胞培养上清中的乳酸脱氢酶(LDH)的活性及细胞内超氧化物歧化酶(SOD)的活性,丙二醛(MDA)的含量。结果黑木耳多1*糖显著提高氧化损伤心肌细胞的存活率,与H2O2损伤组比较,黑木耳多糖各干预组心肌细胞LDH、MDA量显著降低(P0.05),SOD活性显著提高(P0.05),差异有统计学意义;并且黑木耳多糖的抗氧化损伤作用呈剂量依赖关系。结论在体外实验中,黑木耳多糖能减轻H2O2所致的心肌细胞毒性作用,其机制可能与黑木耳多糖有效清除氧自由基,减轻过氧化损伤有关。
[Abstract]:Objective to study the protective effect of Auricularia auricularia polysaccharides on oxidative injury of cardiomyocytes induced by H2O2 and its mechanism.Methods the model of oxidative stress was established by H2O2 induced cardiomyocyte H9c2, which was treated with low, medium and high concentration of Auricularia auricularia polysaccharides.The survival rate of cardiomyocytes was determined by MTT colorimetry. The activity of lactate dehydrogenase (LDHs) and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the supernatant of the cells were detected by the kit.Results the survival rate of myocardial cells damaged by Auricularia auricula was significantly increased by 1 * sugar. Compared with H2O2 injury group, the content of LDHMDA in myocardial cells of Auricularia auricularia polysaccharides intervention group was significantly lower than that of Auricularia auricularia polysaccharide group, and the activity of P0.05 + SOD was increased significantly (P 0.05), and the difference was statistically significant.And the antioxidation effect of Auricularia auricularia polysaccharides was in a dose-dependent manner.Conclusion in vitro, Auricularia auricula polysaccharides can reduce the myocardial cytotoxicity induced by H2O2, and the mechanism may be related to the scavenging effect of Auricularia auricula polysaccharides on oxygen free radicals and reducing the damage of peroxidation.
【作者单位】：新疆医科大学基础医学院;
【基金】：国家自然科学基金(No.81260454)
【分类号】：R151

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