宫内镉暴露对新生儿全基因组DNA甲基化水平的影响

发布时间：2018-04-14 16:05

本文选题：镉 + 环境暴露　；参考：《环境与职业医学》2017年07期

【摘要】：[目的]探讨宫内镉暴露对新生儿全基因组DNA甲基化水平的影响。[方法]选取2011—2012年在某妇幼保健院分娩的300名产妇作为研究对象,分娩时收集新生儿脐动脉血,采用ICP/MS法检测脐动脉血中镉的质量浓度,LC-MS/MS法检测脐动脉血中全基因组DNA甲基化水平。采用Spearman相关分析法和多元线性逐步回归法分析两者之间的关系。[结果]新生儿脐动脉血中镉的质量浓度最小值为0.02μg/L,最大值为2.61μg/L,四分位数为0.08、0.16、0.30μg/L。Spearman相关分析显示,脐动脉血镉的质量浓度与DNA甲基化水平无相关性(r=-0.106,P=0.068)。多元线性逐步回归分析结果显示,排除混杂因素后,脐动脉血镉质量浓度与全基因组DNA甲基化水平呈负相关(P0.05)。[结论]宫内镉暴露可能会使新生儿全基因组DNA甲基化水平降低。
[Abstract]:[objective] to investigate the effect of intrauterine cadmium exposure on DNA methylation in neonates.[methods] 300 parturients who delivered in a maternal and child health care hospital from 2011 to 2012 were selected as the study objects. Umbilical artery blood was collected during delivery.The mass concentration of cadmium in umbilical artery blood was detected by ICP/MS and the whole genome DNA methylation level was detected by LC-MS / MS method.Spearman correlation analysis and multiple linear stepwise regression were used to analyze the relationship between them.[results] the minimum concentration of cadmium in umbilical artery blood was 0.02 渭 g / L, the maximum value was 2.61 渭 g / L, and the quartile was 0.08 渭 g / L and 0.30 渭 g/L.Spearman correlation analysis showed that there was no correlation between the mass concentration of cadmium in umbilical artery blood and DNA methylation level.The results of multiple linear stepwise regression analysis showed that the concentration of cadmium in umbilical artery blood was negatively correlated with the whole genome DNA methylation level after exclusion of confounding factors (P 0.05).[conclusion] Intrauterine cadmium exposure may decrease the level of DNA methylation in neonates.
【作者单位】：杭州医学院公共卫生学院;北京市朝阳区卫生和计划生育监督所;中国疾病预防控制中心环境与健康相关产品安全所;
【基金】：卫生行业科研专项(编号:201002001) 浙江医学高等专科学校博士科研启动项目(编号:2015B06)
【分类号】：R114

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