海地瓜岩藻多糖硫酸酯促进胰岛素抵抗小鼠肝糖原合成及作用机制

发布时间：2018-04-15 12:34

本文选题：海地瓜 + 岩藻聚糖硫酸酯　；参考：《营养学报》2015年04期

【摘要】：目的研究海地瓜岩藻聚糖硫酸酯(Fucoidan isolated from Acaudina molpadioides,Am-FUC)对胰岛素抵抗小鼠肝糖原合成的影响。方法以高脂高糖饲料饲喂雄性C57BL/6J小鼠,建立胰岛素抵抗小鼠模型。模型小鼠饲喂Am-FUC 19w后,检测其空腹血糖、葡萄糖耐受性、胰岛素水平、肝糖原含量及糖代谢关键酶活力。采用q RT-PCR法进一步研究小鼠肝糖原合成通路PKB/GSK-3β中关键基因m RNA表达。结果 Am-FUC能显著降低模型小鼠空腹血糖水平,改善葡萄糖耐受量,提高肝糖原含量;增加肝脏中己糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)等糖原合成相关酶活力,降低糖原磷酸化酶(glycogen phosphorylase,GP)和葡萄糖-6-磷酸酶(glucose-6-phosphatase,G6Pase)等糖异生关键酶活力;显著上调肝脏中IR(insulin receptor,)、IRS-2(insulin receptor substrate-2)、PI3K(phosphatidylinositol 3-hydroxy kinase,)、AKT(Protein Kinase B,)、GS(glycogen synthase)的m RNA及磷酸化蛋白的表达,抑制糖原合成负调节基因GSK-3β(glycogen synthase kinase-3β)的m RNA及磷酸化蛋白的表达。结论 Am-FUC能显著促进模型小鼠合成肝糖原,改善胰岛素抵抗,其作用机制与调控肝脏中糖代谢关键酶活力及PI3K/AKT/GSK-3β糖原合成通路有关。
[Abstract]:Objective to study the effect of Fucoidan isolated from Acaudina molpadioidesa Am-FUCon on hepatic glycogen synthesis in insulin-resistant mice.Methods male C57BL/6J mice were fed with high fat and high sugar diet to establish insulin resistance model.The fasting blood glucose, glucose tolerance, insulin level, liver glycogen content and the key enzyme activity of glucose metabolism were measured in model mice fed with Am-FUC for 19 weeks.The expression of m RNA, a key gene in mouse liver glycogen synthesis pathway PKB/GSK-3 尾, was further studied by Q RT-PCR method.Results Am-FUC could significantly decrease fasting blood glucose level, improve glucose tolerance, increase liver glycogen content, and increase the activities of glycogen synthase related enzymes such as hexokinase (Hexokinase) and pyruvate kinase (pyruvate kinase) in the liver.To inhibit the expression of m RNA and phosphorylated protein of GSK-3 尾 -glycogen synthase kinase-3 尾.Conclusion Am-FUC can significantly promote the synthesis of liver glycogen and improve insulin resistance in model mice. The mechanism is related to the regulation of the activity of key enzymes of glucose metabolism and the PI3K/AKT/GSK-3 尾 glycogen synthesis pathway in the liver.
【作者单位】：中国海洋大学食品科学与工程学院;
【基金】：国家自然科学基金(No.31371876,31471684) 海洋公益性行业科研专项(No.201105029)
【分类号】：R151

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