苯致小鼠遗传损伤与miR-34a表达改变研究

发布时间：2018-04-17 02:22

本文选题：苯 + 苯中毒　；参考：《中国职业医学》2015年02期

【摘要】：目的探讨吸入性苯染毒导致小鼠周围血细胞遗传损伤与miR-34a表达改变的关系。方法无特定病原体级C57BL/6小鼠64只随机分为对照组和低、中、高剂量组,每组16只,雌雄各半;分别给予质量浓度为0.00、3.24、16.22和81.11 mg/m3的苯连续动式吸入染毒,每天染毒6 h。于连续染毒14和28 d时,各组随机抽取雌雄各半共8只小鼠采集全血后处死,检测周围血血常规、骨髓有核细胞与成熟红细胞比值、骨髓嗜多染红细胞微核率和周围血miR-34a相对表达水平等指标。结果高剂量组小鼠白细胞(WBC)和中性粒细胞(NEUT)的计数均低于其余3组(P0.05);中、高剂量组淋巴细胞(LY)和红细胞(RBC)的计数均低于对照组(P0.05),而微核率和miR-34a相对表达水平均高于对照组(P0.05);3个剂量组小鼠LY和RBC的计数均随染毒剂量的增加而下降,微核率和miR-34a相对表达水平均随染毒剂量的增加而上升(P0.05),均呈剂量-效应关系。小鼠血小板计数和骨髓有核细胞与成熟红细胞比值在染毒剂量和染毒时间主效应及两者的交互效应上均无统计学意义(P0.05)。WBC、LY和RBC的计数均与苯染毒剂量呈中度负相关,秩相关系数(rS)分别为-0.598、-0.677和-0.656(P0.01);微核率、miR-34a相对表达水平均与苯染毒剂量呈中度正相关,rS分别为0.790和0.762(P0.01)。微核率和miR-34a相对表达水平分别与WBC、LY和RBC的计数呈负相关,rS分别为-0.543、-0.643、-0.522和-0.562、-0.623、-0.554(P0.01);miR-34a相对表达水平与微核率呈中度正相关,rS为0.617(P0.01)。结论苯致小鼠周围血细胞计数异常和骨髓细胞遗传损伤时,伴随着miR-34a表达的上调。
[Abstract]:Objective to investigate the relationship between the genetic damage of peripheral blood cells and the expression of miR-34a in mice induced by inhaled benzene.Methods Sixty-four C57BL/6 mice of no specific pathogen grade were randomly divided into two groups: control group (n = 16), low dose group (n = 16), middle and high dose group (n = 16, male and female).After 14 and 28 days of continuous exposure, 8 mice of both sexes were randomly selected and killed after collecting whole blood. The peripheral blood routine and the ratio of nucleated bone marrow cells to mature erythrocytes were detected.The micronucleus rate of bone marrow polychromatic erythrocytes and the relative expression level of miR-34a in peripheral blood.Results the counts of WBC and neutrophil neuter in high dose group were lower than those in other three groups (P 0.05).The counts of LY and RBC in the high dose group were lower than those in the control group, while the micronucleus rate and the relative expression level of miR-34a were higher than those in the control group, and the counts of LY and RBC in the three dose groups decreased with the increase of the exposure dose.Both the micronucleus rate and the relative expression level of miR-34a increased with the increase of the exposure dose, which showed a dose-effect relationship.The platelet count and the ratio of nucleated cells to mature red blood cells in mice had no significant correlation with the dose of benzene exposure, the main effect of exposure time and the interaction effect of the two. There was a moderate negative correlation between the counts of P0.05, WBCCU LY and RBC, and the dose of benzene.The rank correlation coefficient (r S) was -0.598tr -0.677 and -0.656 P0.01A, and the relative expression level of micronucleus and benzene exposure dose were 0.790 and 0.762g / P0.01a, respectively.The relative expression level of micronucleus was 0.790 and 0.762g / P0.01a, respectively, and the relative expression level of miR-34a was positively correlated with the dose of benzene exposure.The relative expression level of micronucleus and miR-34a was negatively correlated with the counts of RBC and LY, respectively. The relative expression levels of micronucleus and RBC were -0.543U -0.643C -0.522 and -0.562U -0.623C -0.554A respectively. The relative expression level of miR-34a was moderately positively correlated with the rate of micronucleus.Conclusion the abnormal peripheral blood cell count and genetic damage of bone marrow cells induced by benzene are associated with the up-regulation of miR-34a expression in mice.
【作者单位】：中山大学公共卫生学院;广东省职业病防治院;
【基金】：国家自然科学基金面上项目(81372962,81072284);国家自然科学基金重点项目(81430079)
【分类号】：R114

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