亚慢性砷暴露大鼠红细胞蛋白表达及氧化损伤机制研究

发布时间：2018-04-17 19:37

本文选题：亚慢性 + 砷暴露　；参考：《毒理学杂志》2015年04期

【摘要】：目的观察亚慢性砷暴露对大鼠红细胞蛋白的影响及其氧化损伤的可能机制。方法将健康体重为160~180 g的40只雌雄各半的SD大鼠按体重随机分为对照组,低、中和高剂量砷染毒组共4组,每组按照自由饮水的方法染毒,每组的染毒剂量分别为0、10、60和360μg/L。动物染毒2个月,腹主动脉采血。MEK-6318K全自动血细胞分析仪分析不同组大鼠红细胞参数:红细胞数(RBC)、血红蛋白(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞宽度变异系数(RDW);用还原型谷胱甘肽(GSH)试剂盒采用酶联免疫吸附法(Elisa)测定血浆中的谷胱甘肽含量;采用western-blot的方法分析比较各组大鼠红细胞band-3蛋白和血红蛋白a1链(Hb A1)蛋白质含量。结果与对照组相比,各个染毒组大鼠红细胞参数差异均无统计学意义(P0.05);高剂量砷染毒组band-3和Hb A1含量明显高于对照组,差异有统计学意义(P0.05);中和高剂量砷染毒组的GSH含量明显少于对照组和低剂量砷染毒组,差异有统计学意义(P0.05)。结论亚慢性砷暴露可以引起大鼠红细胞蛋白含量差异及氧化损伤。
[Abstract]:Objective to observe the effect of subchronic arsenic exposure on erythrocyte protein and the possible mechanism of oxidative damage in rats.Methods Forty male and female Sprague-Dawley rats with a healthy weight of 180 g were randomly divided into 4 groups according to their body weight: low, medium and high dose arsenic exposure groups. Each group was treated with free drinking water, the doses of each group were 0 ~ 10g / L and 360 渭 g 路L ~ (-1), respectively.Animals were poisoned for 2 months.Analysis of erythrocyte parameters in different groups by automatic Hematology Analyzer .MEK-6318K: RBC number RBCU, HGBM, HCT, mean RBC Volume MCV, mean RBC hemoglobin content MCHC, mean RBCThe erythrocyte width variation coefficient (RDWN) of hemoglobin concentration was determined by enzyme-linked immunosorbent assay (Elisa) with reduced glutathione glutathione (GSH) kit, and the plasma glutathione content was determined by enzyme-linked immunosorbent assay (Elisa).The contents of red blood cell band-3 protein and hemoglobin a 1 chain HbA1 protein were analyzed and compared by western-blot.Results compared with the control group, there was no significant difference in erythrocyte parameters between the two groups, and the contents of band-3 and HB A1 in the high dose arsenic group were significantly higher than those in the control group.The content of GSH in the middle and high dose arsenic groups was significantly lower than that in the control group and the low dose arsenic group, and the difference was statistically significant.Conclusion Subchronic arsenic exposure can induce the difference of erythrocyte protein content and oxidative damage in rats.
【作者单位】：山西医科大学公共卫生学院卫生毒理教研室;山西省太原市晋源区人民政府;
【基金】：山西省科技基础条件平台建设项目(2011091005-0102) 山西省回国人员基金(200945) 山西省科技攻关项目(2006031087-11)
【分类号】：R114

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