二硫化碳染毒对大鼠肾脏的影响

发布时间：2018-04-18 00:33

本文选题：二硫化碳 + 大鼠　；参考：《重庆医学》2017年18期

【摘要】：目的探讨二硫化碳(CS_2)染毒对大鼠肾脏的损害特点及其可能的发生机制。方法 80只SD大鼠随机分为对照组(A组)及0、50、150、250mg/kg CS_2染毒组(B、C、D、E组),每组16只。分别在染毒12、16周末两个时间点留取每组6只大鼠的尿液,检测尿蛋白/肌酐、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG);留取血液检测肾功能;观察各组大鼠肾脏病理改变、肾小管上皮细胞凋亡及肾脏Bax蛋白表达情况。结果在12、16周末,大鼠尿NAG活性在各染毒组明显增加(P0.05),且随CS_2染毒剂量增加而增加;尿蛋白/肌酐无明显变化(P0.05)。16周末血尿素氮(BUN)和肌酐水平在D、E组均升高,且E组升高更明显(P0.05)。HE染色结果显示CS_2染毒主要损害肾小管,呈局灶性颗粒空泡变性,呈剂量-效益关系。肾小管上皮细胞凋亡数目随CS_2染毒剂量增加和染毒时间延长而增加,凋亡细胞在12周末时主要出现在近端小管上皮细胞,而16周末时则主要出现在远端小管上皮细胞。免疫组化显示随染毒剂量增加和染毒时间延长,染毒组肾小管上皮细胞质Bax蛋白染色程度加深及面积增大。结论 CS_2染毒可导致大鼠肾脏结构和功能的损害,细胞凋亡可能是CS_2致肾脏损害的机制之一。
[Abstract]:Objective to investigate the characteristics and possible mechanism of kidney damage induced by carbon disulfide (CS2) in rats.Methods 80 Sprague-Dawley rats were randomly divided into control group (n = 16) and control group (n = 16).Urine samples of 6 rats in each group were collected at two time points at the end of the 12th week of exposure, urine protein / creatinine, urine Nacetyl 尾 -Daminoglycosidase (Nacetyl 尾 -Dglucosaminidase) was detected, blood was taken to detect renal function, and pathological changes of kidney were observed in each group.Apoptosis of renal tubular epithelial cells and expression of renal Bax protein.Results at the end of the 12th week, the urinary NAG activity of rats increased significantly in each exposure group, and increased with the increase of CS_2 dose, while the urinary protein / creatinine ratio did not change significantly at the end of week 16. The level of blood urea nitrogen bun and creatinine increased in Dale group at the end of the week.The results of P0.05A and HE staining showed that the renal tubules were mainly damaged by CS_2 exposure, with a dose-benefit relationship.The number of apoptosis of renal tubule epithelial cells increased with the increase of CS_2 exposure dose and exposure time. The apoptotic cells mainly appeared in proximal tubule epithelial cells at the 12th week and distal tubule epithelial cells at the 16th week.Immunohistochemical staining showed that the degree and area of Bax protein in renal tubular epithelial cytoplasm increased with the increase of dose and time.Conclusion CS_2 exposure may lead to the damage of renal structure and function in rats. Apoptosis may be one of the mechanisms of renal damage induced by CS_2.
【作者单位】：重庆市第五人民医院肾病内科;西南医科大学附属医院肾病内科;西南医科大学附属医院病理科;
【分类号】：R135.1

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