纳米氧化铈对过氧化氢所致大鼠肺泡巨噬细胞氧化损伤的影响

发布时间：2018-04-18 04:18

本文选题：纳米氧化铈 + 大鼠肺泡巨噬细胞　；参考：《环境与健康杂志》2017年01期

【摘要】：目的研究纳米氧化铈对过氧化氢致大鼠肺泡巨噬细胞NR8383氧化损伤的影响。方法将对数生长期的NR8383细胞分别暴露于终浓度为5、10、20、50、100μg/ml纳米氧化铈(20 nm)悬液孵育24 h,再加入新鲜配制的终浓度为100μmol/L的过氧化氢刺激细胞2 h,另设对照(PBS)组及过氧化氢(100μmol/L)组。采用CCK-8法检测NR8383细胞的细胞活性,采用酶标法检测培养液上清中乳酸脱氢酶(LDH)的释放量,并测定细胞内谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、活性氧(ROS)的水平。结果与对照组相比,过氧化氢对NR8383细胞造成氧化损伤,细胞的存活率下降25.25%。与过氧化氢组相比,5μg/ml纳米氧化铈+过氧化氢组NR8383细胞的存活率较高,而100μg/ml纳米氧化铈+过氧化氢组NR8383细胞的存活率较低,差异均有统计学意义(P0.05,P0.01);且随着纳米氧化铈染毒浓度的升高,NR8383细胞的存活率呈下降的趋势。与过氧化氢组相比,对照组NR8383细胞的LDH释放量较低,50μg/ml纳米氧化铈+过氧化氢组NR8383细胞的LDH释放量较高,差异有统计学意义(P0.05);而5、10μg/ml纳米氧化铈+过氧化氢组NR8383细胞的LDH释放量有所降低,但差异均无统计学意义(P0.05)。且随着纳米氧化铈染毒浓度的升高,NR8383细胞的LDH释放量呈上下波动。与过氧化氢组相比,对照组NR8383细胞内GSH的水平升高,而ROS的水平上升;5、10μg/ml纳米氧化铈+过氧化氢组NR8383细胞内的ROS水平均下降,而10μg/ml纳米氧化铈+过氧化氢组NR8383细胞内的GSH水平上升,差异均有统计学意义(P0.05)。随着纳米氧化铈染毒浓度的升高,NR8383细胞内的SOD、GSH呈上下波动,ROS的水平呈逐渐升高的趋势。结论低浓度(5、10μg/ml)的纳米氧化铈颗粒可以缓解过氧化氢对NR8383细胞造成的氧化损伤,对细胞起到保护作用。
[Abstract]:Objective to study the effect of nano cerium oxide on oxidative damage of NR8383 in rat alveolar macrophages induced by hydrogen peroxide.Methods the NR8383 cells in logarithmic growth phase were exposed to 50 渭 mol / L C2O _ 3 (50 渭 mol / L) suspension for 24 h, then incubated with hydrogen peroxide (100 渭 mol/L) for 2 h. The cells were divided into two groups: control group (n = 10) and hydrogen peroxide group (n = 100 渭 mol / L).The activity of NR8383 cells was detected by CCK-8 assay, the release of lactate dehydrogenase (LDH) from supernatant of culture medium was detected by enzyme labeling method, and the levels of glutathione glutathione (GSH), superoxide dismutase (SOD) and reactive oxygen species (Ros) were measured.Results compared with the control group, hydrogen peroxide caused oxidative damage to NR8383 cells and the survival rate of cells decreased 25.25%.Compared with hydrogen peroxide group, the survival rate of 5 渭 g/ml nano-cerium peroxide group was higher than that of 100 渭 g/ml nano-cerium oxide hydrogen peroxide group, while the survival rate of 100 渭 g/ml nano-cerium oxide group was lower than that of hydrogen peroxide group.The difference was statistically significant, and the survival rate of NR8383 cells decreased with the increase of the concentration of cerium oxide nanoparticles.Compared with the hydrogen peroxide group, the LDH emission of NR8383 cells in the control group was lower than that in the 50 渭 g/ml nano-cerium oxide hydrogen peroxide group, and the LDH release of the NR8383 cells in the control group was higher than that in the hydrogen peroxide group.The difference was statistically significant (P 0.05), while the release of LDH was decreased in 5 渭 g / 10 渭 g/ml hydrogen peroxide group, but there was no significant difference in LDH emission between the two groups (P 0.05).The amount of LDH released from NR8383 cells fluctuated with the increase of the concentration of cerium oxide nanoparticles.Compared with hydrogen peroxide group, the level of GSH in NR8383 cells of control group was increased, while the level of ROS in NR8383 cells of 10 渭 g/ml nano-cerium oxide group decreased.However, the level of GSH in NR8383 cells increased in 10 渭 g/ml nano-cerium oxide hydrogen peroxide group, and the difference was statistically significant (P 0.05).With the increase of the concentration of cerium oxide in NR8383 cells, the level of GSH in NR8383 cells fluctuated up and down, and the level of Ros increased gradually.Conclusion low concentration of 10 渭 g / ml cerium oxide nanoparticles can alleviate the oxidative damage caused by hydrogen peroxide on NR8383 cells and protect the cells.
【作者单位】：内蒙古科技大学包头医学院基础医学与法医学院;
【基金】：国家自然科学基金(81160341) 内蒙古自治区自然科学基金(2015MS0868)
【分类号】：R114

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