重庆市某区小学女生多环芳烃内暴露水平及其与青春发动时相的关系

发布时间：2018-04-19 05:18

本文选题：多环芳烃 + 高效液相色谱　；参考：《卫生研究》2017年05期

【摘要】：目的建立同时测定尿中4种多环芳烃(PAHs)代谢物的检测方法,研究重庆市某区女童青春发动时相提前与体内多环芳烃暴露水平的关系。方法研究对象采用目的性抽样,对重庆市某区4所小学1~4年级女生进行一般情况的问卷调查、生长发育的体格检查、并收集尿液,采用高效液相色谱-质谱联用法(HPLC-MS)对样品中4种PAHs代谢物进行定性和定量检测。结果 4种PAHs代谢物标准曲线相关性高,方法检出限为0.1 ng/mL。研究共调查女童737名,青春发动时相提前组209人,正常组528人。尿液检测结果显示4种PAHs代谢物检出率为100%,4种代谢物的检出浓度范围分别为1-羟基芘0.01~4.77 ng/mL,2-羟基萘0.15~50.00ng/mL,2-羟基芴0.06~12.59 ng/mL及9-羟基菲0.29~23.17 ng/mL。青春发动时相提前组和正常组在2-羟基芴(Z=-1.996)和9-羟基菲(Z=-3.161)暴露水平上差异具有统计学意义(P0.05),控制了肥胖因素后,青春发动时相提前组9-羟基菲(Z=-3.012)暴露水平仍高于正常组(P0.05)。结论该方法适用于4种PAHs代谢物的同时检测,研究地区女童青春发育早期均有PAHs暴露,且PAHs暴露可能是女童青春发动时相提前的因素之一。
[Abstract]:Objective to establish a method for simultaneous determination of four polycyclic aromatic hydrocarbons (PAHs) metabolites in urine, and to study the relationship between the early onset phase and the exposure level of polycyclic aromatic hydrocarbons (PAHs) in the body of girls in a certain district of Chongqing.Methods the female students of grade 1 and grade 4 in four primary schools in Chongqing were investigated by means of purposeful sampling, and their growth and development were examined, and urine was collected.Four PAHs metabolites in the samples were determined qualitatively and quantitatively by high performance liquid chromatography-mass spectrometry (HPLC-MS).Results the standard curves of four PAHs metabolites were highly correlated, and the detection limit was 0.1 ng / mL.A total of 737 girls were investigated, 209 of them were in the early stage of youth initiation and 528 in the normal group.Urine tests showed that the detectable concentrations of four metabolites of PAHs were 100 and 0.01ng / mL of 1-hydroxypyrene and 4.77ng / mL, respectively. The concentration of 2-hydroxynaphthalene was 0.15ng- 50.00ng / mL- 2-hydroxylfluorene (0.06nm) 12.59 ng/mL, and the concentration of 9-hydroxyphenanthrene 0.2923.17ng/ mL 路mL ~ (-1).There was significant difference in the exposure levels of 2-hydroxyfluorene Z-1.996) and 9- hydroxyphenanthrene Z-3.161) between the early stage group and the normal group. After controlling for the obesity factors, the exposure level of 9-hydroxyphenanthrene Z-3.012 in the early phase group was still higher than that in the normal group.Conclusion this method is suitable for simultaneous detection of four PAHs metabolites. PAHs exposure may be one of the factors leading to early onset of youth in girls in the study area.
【作者单位】：重庆医科大学公共卫生与管理学院医学与社会发展研究中心健康领域社会风险预测治理协同创新中心;
【基金】：国家自然科学基金青年科学基金(No.81502825) 重庆市科学技术委员会基础与前沿项目(No.cstc2013jcyj A10001)
【分类号】：R179

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