碳-12重离子诱导正常人疱疹病毒-转化淋巴细胞非稳定性染色体畸变研究

发布时间：2018-04-19 09:35

本文选题：重离子 + 碳-　；参考：《中国职业医学》2015年01期

【摘要】：目的探讨碳-12(12C)重离子诱导正常人疱疹病毒(EBV)-转化淋巴细胞染色体畸变的生物效应。方法以照射能量为165 Me V/u,剂量率为0.3~0.5 Gy/min,照射剂量分别为0.0、0.1、0.3、0.5、0.7、1.0和2.0 Gy的12C重离子来辐照对数增长期正常人EBV-淋巴细胞,分别培养至照后48、72 h收获细胞(分别设为48和72 h组),收获前2 h加秋水仙素,制片,计算染色体畸变细胞率、"双+环"畸变率以及总非稳定性畸变率,并拟合剂量-效应回归方程。结果 48 h组的"双+环"畸变率在0.3~2.0 Gy约为72 h组的2倍。2组染色体畸变细胞数量均随照射剂量的增加而增加,呈剂量-效应关系(P0.01)。48 h组的畸变细胞率、"双+环"畸变率以及非稳定性畸变率在0.0~2.0 Gy的剂量-效应回归方程分别为Y=0.024+0.708 D-0.175 D2[校正决定系数(R2c)=0.963,P0.01]、Y=-0.034+0.531 D-0.128 D2(R2c=0.950,P0.01)和Y=0.009+0.868 D-0.089 D2(R2c=0.980,P0.01);72 h组的上述指标分别为Y=0.039+0.388 D-0.084 D2(R2c=0.976,P0.01)、Y=-0.013+0.247 D-0.062 D2(R2c=0.900,P0.01)和Y=0.034+0.490 D-0.046 D2(R2c=0.987,P0.01)。结论12C重离子诱导正常人EBV-转化淋巴细胞畸变随照射剂量增加而增加;染色体畸变在0.0~2.0 Gy存在一定剂量-效应关系,符合二次多项式回归模型。
[Abstract]:Objective to investigate the biological effects of heavy ion induced chromosomal aberration of normal human herpesvirus (HHV)-transformed lymphocytes.Methods EBV- lymphocytes in logarithmic growth phase were irradiated with 165Me V / u, dose rate of 0.3g / min and dose of 0.00.1g / min, respectively, and 12C heavy ions with a dose of 0.71.0Gy and 2.0 Gy, respectively, in logarithmic growth phase of human EBV- lymphocytes.The cells were cultured to 48 ~ 72 h after irradiation (48 h and 72 h, respectively), and colchicine was added 2 hours before harvest. The rate of chromosome aberration cells, the rate of "double loop" aberration and the rate of total unstable aberration were calculated.The dose-effect regression equation was fitted.Results the rate of "double loop" aberration in 48 h group was about 2 times of that in 72 h group. The number of chromosome aberration cells increased with the increase of irradiation dose.The dose-effect regression equations of "double loop" aberration rate and unsteady aberration rate at 0.00 ~ 2 Gy were: YP0.024 0.708 D-0.175D2 [calibration decision coefficient R2cCU 0.963g P0.01] Y-0.034 0.531 D-0.128D2C2C2C0.950 P0.01) and YY 0.009 0.868 D-0.089 D2OR2CU 0.980P0.01h for 72 h.The above indexes of the two groups were: YP0.039 0.388 D-0.084 D2CU 0.976U P0.01P0.013 0.247 D-0.062 D2C0.9000P0.01) and YP0.034 0.490 D-0.046D2OR2CU 0.987P0.01.Conclusion 12C heavy ion induced EBV- transformed lymphocyte aberration increases with the increase of irradiation dose, and chromosome aberration has a dose-effect relationship at 0.020 Gy, which accords with the quadratic polynomial regression model.
【作者单位】：中国辐射防护研究院;
【分类号】：R144

【参考文献】