2011-2014年福建省手足口病相关病原柯萨奇病毒A组4型的分子流行病学分析

发布时间：2018-04-21 03:36

本文选题：手足口病 + 柯萨奇病毒A　；参考：《中国人兽共患病学报》2017年09期

【摘要】：目的分析福建省手足口病患者中柯萨奇病毒A组4型(Coxsackievirus A4,CVA4)的流行病学、基因变异及遗传进化等特征。方法应用细胞培养方法获得病毒分离株,RT-PCR方法进行分子分型及扩增CVA4血清型完整VP1区全长序列,并进行序列变异及遗传进化分析。结果 2011-2014年,从福建省手足口病其它肠道病毒病例标本中分子分型33例CVA4病例,在407例的其它肠道病毒病例中的构成比为8.1%,其中2012年31例,2014年2例。CVA4病例在性别、年龄组分布特征,与一般手足口病流行特征相比,没有特异性。VP1区序列差异比较表明,2011-2014年福建省CVA4分离株之间VP1核苷酸及氨基酸序列相似性分别在92.6%~100%、95.7%~100%;与原型株及国外地方株的核苷酸序列相似性较低,分别为83.7%~85.4%、82.1%~89.1%,氨基酸序列相似性分别为96.1%~99.0%、90.4%~99.6%;与国内地方株核苷酸及氨基酸序列相似性较高,分别为87.9%~99.2%、96.1%~100%。进化树结果表明福建CVA4分离株与原型株及国外代表株的遗传距离远,与国内代表株的遗传距离近。福建CVA4分离株在进化树的分布呈多个分支。结论 CVA4已成为近年来福建省除EV71、CVA16、CVA6、CVA10外导致手足口病的又一重要病原体。福建CVA4分离株与国内地方株同进化共循环,在福建省各个地区同时流行多条亲缘关系接近的传播链。
[Abstract]:Objective to analyze the epidemiology, genetic variation and genetic evolution of coxsackievirus A group 4 (Coxsackievirus A4) in patients with hand, foot and mouth disease (HFMD) in Fujian Province. Methods the full-length VP1 region of CVA4 serotype was amplified by RT-PCR with cell culture method, and the sequence variation and genetic evolution were analyzed. Results from 2011 to 2014, 33 cases of CVA4 were classified from other enterovirus specimens of hand, foot and mouth disease in Fujian Province, and the constituent ratio of other enterovirus cases was 8.1 in 407 cases, including 31 cases in 2012 and 2 cases in 2014. Compared with the epidemic characteristics of hand, foot and mouth disease, the distribution of age group, There was no specific sequence difference in VP1 region. The results showed that the similarity of nucleotide and amino acid sequence of VP1 among CVA4 isolates in Fujian Province from 2011 to 2014 was 92.6% and 95.775%, respectively, and the nucleotide sequence similarity was low with the prototype strain and foreign local strain. The similarity of amino acid sequence was 96.1 and 99.0, 99.0 and 99.6, respectively, and the similarity of nucleotide and amino acid sequence with local strains was 87.9 and 99.21, respectively. The results of phylogenetic tree showed that the genetic distance between Fujian CVA4 isolates and prototypes and foreign representative strains was long and close to that of domestic representative strains. The distribution of Fujian CVA4 isolates in phylogenetic tree showed multiple branches. Conclusion CVA4 has become an important pathogen of HFMD in Fujian Province in recent years, in addition to EV71, CVA16, CVA6, CVA10. The CVA4 isolates of Fujian and the local strains in China have the same evolution and cocirculation, and there are many transmission chains with close genetic relationship in different areas of Fujian Province.
【作者单位】：福建省疾病预防控制中心;
【基金】：福建省自然科学基金(No.2016J01350) 福建省卫计委青年科研课题(No.2014-1-25)联合资助~~
【分类号】：R181.3;R725.1

【相似文献】