低浓度苯暴露对大鼠神经行为的影响

发布时间：2018-04-23 09:55

本文选题：苯 + 吸入染毒　；参考：《现代预防医学》2015年23期

【摘要】：目的研究低浓度气态苯吸入对大鼠学习记忆等神经行为的影响,并探讨低浓度苯吸入对大鼠血象的改变。方法SD雄性大鼠随机分4组,每组10只,低、中、高剂量组苯染毒浓度分别为4 mg/m3、6 mg/m3和10 mg/m3,对照组吸入空气。采用静式吸入染毒法,4 h/d,5 d/w,持续染毒4 w,染毒结束后进行学习记忆等神经行为学测试、血象、嗜多染红细胞微核实验。结果 Morris水迷宫定位航行实验,高剂量组大鼠d 3、d 4的逃避潜伏期明显高于对照组(P0.05),空间探索实验的目标象限时间明显低于对照组(P0.05)。高剂量组大鼠的后肢撑力指数明显高于对照组(P0.05)。低、中、高剂量组白细胞计数明显低于对照组(P0.05)。结论低剂量、短时间暴露苯可损害大鼠学习记忆及运动功能,引起外周血白细胞计数降低。
[Abstract]:Objective to study the effects of low concentration gaseous benzene inhalation on learning and memory of rats, and to explore the changes of blood picture in rats after low concentration benzene inhalation. Methods SD male rats were randomly divided into 4 groups with 10 rats in each group. The concentration of benzene in the low, middle and high dose groups was 4 mg / m ~ (3) mg/m3 and 10 mg / m ~ (-3) respectively. The control group inhaled air. A static inhalation method was used for 4 h / d 5 d / w, and 4 weeks of continuous exposure. After the exposure, neurobehavioral tests, such as learning and memory tests, hemogram, polychromatic erythrocyte micronucleus test were carried out. Results in the Morris water maze navigation experiment, the escape latency of d 3 d 4 in the high dose group was significantly higher than that in the control group (P 0.05), and the target quadrant time of the space exploration experiment was significantly lower than that of the control group (P 0.05). The hind limb support index in high dose group was significantly higher than that in control group (P 0.05). The white blood cell count in the low, middle and high dose groups was significantly lower than that in the control group (P 0.05). Conclusion low dose and short exposure to benzene can impair the learning, memory and motor function of rats and decrease the leukocyte count in peripheral blood.
【作者单位】：华北理工大学公共卫生学院劳动卫生与环境卫生学科/河北省煤矿卫生与安全实验室;大连市健康教育中心;
【基金】：河北省科技计划项目(13277709D)
【分类号】：R114

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