孕哺期大鼠全氟辛烷磺酸暴露对子代糖代谢的影响

发布时间：2018-04-24 10:04

本文选题：全氟辛烷磺酸 + 糖代谢　；参考：《环境与职业医学》2015年03期

【摘要】：[目的]探讨孕哺期全氟辛烷磺酸(PFOS)暴露对子代大鼠糖代谢的影响。[方法]将Wistar孕鼠自孕0天(GD0)随机分为对照组(0 mg/kg)、低剂量组(0.6 mg/kg)和高剂量组(2 mg/kg),每组10只;PFOS灌胃染毒至仔鼠出生后21天(PND21)断乳为止。采用高效液相/质谱法检测PND0、PND21时仔鼠血清PFOS含量;观察仔鼠体重变化趋势;比较9周龄和15周龄仔鼠空腹血糖、空腹胰岛素水平;检测仔鼠瘦素和抵抗素基因表达水平变化。[结果]PND21时低剂量组和高剂量组仔鼠血清中PFOS浓度分别为(16.00±1.27)mg/L和(80.54±6.55)mg/L(P0.05)。低剂量组雌性仔鼠出生后8、9周龄和高剂量7~9周龄,低剂量组雄性仔鼠出生8~12周龄、高剂量7、8、10周龄的体重均明显低于对照组(均P0.05)。高剂量组9周龄和低剂量组15周龄雌性仔鼠的胰岛素水平分别为(10.85±1.37)m U/L和(13.62±1.87)m U/L,均高于对照组(P0.05);高剂量组9周龄雄性仔鼠的空腹血糖和胰岛素水平分别为(5.43±0.77)mmol/L和(13.23±1.81)m U/L,15周龄雄性仔鼠低剂量组分别为(4.99±0.54)mmol/L和(13.57±1.22)m U/L,15周龄高剂量组分别为(5.71±0.56)mmol/L和(13.44±2.97)m U/L,均高于对照组(P0.05)。高剂量组15周龄雄性仔鼠的抵抗素基因表达上调1.25±0.03(P0.05),瘦素基因表达下调0.67±0.08(P0.05)。[结论]PFOS孕哺期暴露可能引起子代大鼠糖代谢异常,增加糖尿病患病风险。
[Abstract]:[objective] to investigate the effect of PFOS exposure on glucose metabolism in offspring rats. [methods] Wistar pregnant rats were randomly divided into control group (0 mg / kg), low dose group (0.6 mg / kg) and high dose group (2 mg / kg). High performance liquid phase / mass spectrometry (HPLC / MS) was used to detect serum PFOS content, body weight change trend, fasting blood glucose and fasting insulin level and leptin and resistin gene expression in neonatal rats at 9 and 15 weeks of age. [results] the serum PFOS concentrations in the low dose group and the high dose group were 16.00 卤1.27)mg/L and 80.54 卤6.55 mg / L respectively at PND21. The body weight of the female rats in the low dose group was significantly lower than that in the control group at the age of 8 ~ 9 weeks after birth and that of the male mice in the low dose group at the age of 8 ~ 12 weeks after birth (P < 0.05), and the body weight of the male mice in the low dose group was significantly lower than that in the control group at the age of 8 ~ 12 weeks (P < 0.05). The insulin levels of female rats at the age of 9 weeks in the high dose group and 15 weeks in the low dose group were 10.85 卤1.37 渭 / L and 13.62 卤1.87 渭 / L, respectively, which were higher than those in the control group (P0.05), and the fasting blood glucose and insulin levels of the 9-week-old male rats in the high-dose group were 5.43 卤0.77)mmol/L and 13.23 卤1.81mUL / L, respectively, and were 13.23 卤1.81mUL / L for 15 weeks, respectively. The low dose group (4.99 卤0.54)mmol/L) and the high dose group (13.57 卤1.22mU / L) were 5.71 卤0.56)mmol/L and 13.44 卤2.97mU / L, respectively, which were higher than those of the control group (P 0.05). In high dose group, resistin gene expression was up-regulated by 1.25 卤0.03P0.05 and leptin gene expression was down-regulated by 0.67 卤0.08P0.05in 15-week-old male rats. [conclusion] exposure to PFOS during pregnancy and feeding may lead to abnormal glucose metabolism in offspring and increase the risk of diabetes mellitus.
【作者单位】：新疆医科大学公共卫生学院;南通大学公共卫生学院;
【基金】：国家自然科学基金(编号:81202228) 南通市自然科学基金项目(编号:HS2012012) 南通大学大学生创新训练计划项目(编号:2014104) 南通大学教学改革课题(编号:2013B026)
【分类号】：R114

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