生活饮用水中非苛养耐药菌的污染研究

发布时间：2018-04-24 14:57

本文选题：自来水 + 非苛养细菌　；参考：《军事医学》2017年08期

【摘要】：目的调查天津某地小区生活饮用水中非苛养耐药菌的污染状况。方法采用R2A琼脂对自来水进行细菌分离,利用16S rRNA基因测序法对细菌分离株进行种属鉴定。同时采用Kirby-Bauer纸片扩散法(K-B纸片法)进行细菌的抗生素敏感性试验。结果在分离的39株非苛养菌中,耐药菌占79.49%,耐药细菌涉及肠球菌属、葡萄球菌属、不动杆菌属、假单胞菌属。其中,仅对一种抗生素耐药的细菌占28.21%,而多药耐药菌则占51.28%。耐药菌对复方新诺明的耐药最为普遍,耐药率为53.85%;对磺胺甲VA唑的耐药次之,占28.21%。结论该小区生活饮用水中的非苛养耐药菌污染较为严重,应加强关注其潜在的人群风险。
[Abstract]:Objective to investigate the contamination of non-resistant bacteria in drinking water in Tianjin. Methods R2A Agar was used to isolate bacteria from tap water and 16s rRNA gene sequencing method was used to identify the species of bacteria isolated from tap water. At the same time, Kirby-Bauer disk diffusion method and K B disk method were used to test the antibiotic sensitivity of bacteria. Results among the 39 strains of non-caustic culture bacteria, resistant bacteria accounted for 79.49. The resistant bacteria involved Enterococcus, Staphylococcus, Acinetobacter, Pseudomonas. Among them, only one antibiotic resistant bacteria accounted for 28. 21%, and multi-drug resistant bacteria accounted for 51. 28%. The resistance of drug-resistant bacteria to compound sulfamethoxazole was the most common, and the resistance rate was 53.85, followed by sulfamethazol (28.21%). Conclusion the contamination of non-resistant bacteria in drinking water in this district is serious, so we should pay more attention to the potential population risk.
【作者单位】：军事医学科学院卫生学环境医学研究所;
【基金】：国家自然科学基金面上项目(81372947)
【分类号】：R123

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