硒蛋白Se-76P对STZ诱导的小鼠糖尿病肾病模型肾功能及生化指标的影响

发布时间：2018-04-25 15:02

本文选题：硒蛋白 + Se-P　；参考：《食品工业科技》2017年14期

【摘要】：研究含硒蛋白(Se-76P)对链脲佐菌素(STZ)诱导的小鼠糖尿病肾病的保护作用。C57BL/6J雄性小鼠腹腔注射STZ(40 mg/kg)制备糖尿病肾病模型,将60只小鼠分为六组,正常对照组(Ⅰ组)、糖尿病肾病模型组(Ⅱ组)、模型+Se-76P低剂量组(Ⅲ组)、模型+Se-76P中剂量组(Ⅳ组)、模型+Se-76P高剂量组(Ⅴ组)和模型+胰岛素治疗组(Ⅵ组)。各组小鼠均采用皮下注射方式给药,Ⅰ组和Ⅱ组给予生理盐水;Ⅲ、Ⅳ、Ⅴ组分别给予低(20μmol/kg)、中(40μmol/kg)、高(60μmol/kg)浓度Se-76P蛋白;Ⅵ组给予5 U/kg·d胰岛素。各组连续给药4周,每周测量小鼠体重;实验结束后检测各组小鼠空腹血糖、血清肌酐(SCr)和尿素氮(BUN)水平;检测各组小鼠尿肌酐(UCr)和尿微量白蛋白(MAU)水平,并计算肌酐清除率(CCr);检测肾组织丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)水平。与模型组相比,Se-76P各治疗组空腹血糖值均降低;SCr、BUN、UCr、MAU水平以及CCr均显著降低(p0.01或0.05);肾组织中MDA水平明显降低(p0.01),而SOD和GPx活性明显升高(p0.01或0.05)。Se-76P可能通过提高SOD和GPx活性,提升抗氧化水平,改善STZ诱导的糖尿病肾病小鼠模型的肾功能。
[Abstract]:The protective effect of selenoprotein (Se-76P) on diabetic nephropathy induced by streptozotocin (STZ) induced diabetic nephropathy in.C57BL/6J mice was intraperitoneally injected with STZ (40 mg/kg) to prepare diabetic nephropathy model. 60 mice were divided into six groups, normal control group (group I), diabetic nephropathy model group (Group II), model +Se-76P low dose group (Group III), model +Se-76P Medium dose group (Group IV), model +Se-76P high dose group (Group V) and model + insulin treatment group (Group VI). Mice in each group were given subcutaneous injection, group I and group II were given saline, group III, IV, and V were given low (20 u mol/kg), medium (40 mol/ kg), high (60 mu mol/kg) concentration Se-76P protein, and group VI was given 5 U/kg. D insulin. The body weight of mice was measured weekly for 4 weeks. After the experiment, the fasting blood glucose, serum creatinine (SCr) and urea nitrogen (BUN) levels were detected in each group. The serum creatinine (UCr) and urinary microalbumin (MAU) levels were measured and the creatinine clearance rate (CCr) was measured, and the renal tissue malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione were detected. Peroxidase (GPx) level. Compared with the model group, the fasting blood glucose levels in each group of Se-76P were decreased, the levels of SCr, BUN, UCr, MAU, and CCr were significantly decreased (P0.01 or 0.05), and the MDA level in the renal tissue decreased significantly (P0.01), while SOD and GPx activity increased significantly (or 0.05). Objective to improve the renal function of STZ induced diabetic nephropathy mice.

【作者单位】：吉林医药学院检验学院免疫技术教研室;吉林医药学院临床医学院医学影像系;吉林医药学院基础医学院生物化学教研室;
【基金】：吉林省科技厅自然科学基金项目(20150101131JC) 吉林省教育厅“十二五”科学技术研究项目(吉教合字2015-405) 吉林省卫生厅科技计划基金资助项目(2013ZC015)
【分类号】：R151

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