功夫菊酯抑制雌激素对小鼠海马突触发育促进作用的体外研究

发布时间：2018-04-25 18:30

本文选题：海马 + 神经元　；参考：《中华疾病控制杂志》2015年07期

【摘要】：目的探讨功夫菊酯(lambda-cyhalothrin,LCT)是否因其拟雌激素作用而影响小鼠海马神经突触发育。方法取孕18 d的ICR小鼠胚胎海马神经细胞体外培养7 d,用LCT(0.1、1、10μmol/L)、雌激素(10 nmol/L)、LCT(10μmol/L)加雌激素(10 nmol/L)、LCT(10μmol/L)加ICI182 780(ERα抑制剂,1μmol/L)、雌激素(10 nmol/L)加ICI182 780(1μmol/L)处理24 h,CCK-8法检测细胞活性,免疫荧光细胞化学法观察神经元突起发育和突触后致密蛋白95(post-synaptic density protein 95,PSD95)。结果 LCT或雌激素单独处理可增加海马神经元突起的数目(F=18.16,P0.001)和长度(F=12.23,P0.001)以及PSD95的表达(F=12.71,P0.001),雌激素受体抑制剂ICI182,780可以阻断该作用(F=12.71,P0.001);LCT与雌激素联合作用干扰了雌激素对神经元突起数目(F=18.16,P0.001)和PSD95表达的促进作用(F=12.71,P0.001)。结论 LCT可抑制雌激素对神经突触发育的促进作用。
[Abstract]:Aim to investigate whether lambda-cyhalothrinin (LCTT) affects the development of hippocampal synapses in mice due to its estrogenic effect. Methods Hippocampal neurons of 18 day pregnant ICR mice were cultured in vitro for 7 days. The cell activity was measured by 24 h CCK-8 treatment with 10 nmol / L estradiol 10 nmol / L LCTT + 10 nmol / L estradiol 10 渭 mol / L) plus 1 渭 mol / L ICI182 780(ER 伪 inhibitor, 10 nmol / L estradiol) plus ICI182 7801 渭 mol / L) at 24 h after treatment with 10 渭 mol / L, 10 渭 mol / L). The neurite development and postsynaptic dense protein 95(post-synaptic density protein 95 were observed by immunofluorescence cytochemistry. Results LCT or estrogen alone could increase the number of neuronal processes in hippocampal neurons (F _ (18.16) P _ (0.001) and F _ (12.23) P _ (0.001), as well as the expression of PSD95. Estrogen receptor inhibitor ICI182780 could block this effect. The number of protrusions and the role of PSD95 in promoting the expression of P0.001 and the number of protuberances in the cells were increased by 12.71% of P0.001 and 12.71% of P0. 001, respectively. Conclusion LCT can inhibit the effect of estrogen on neurosynaptic development.
【作者单位】：安徽医科大学公共卫生学院卫生毒理学系;
【基金】：国家自然科学基金(30571585)
【分类号】：R114

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