基于16S rDNA序列的1株灭螺微生物的分子鉴定

发布时间：2018-04-26 19:22

本文选题：钉螺 + 微生物　；参考：《江苏农业科学》2017年01期

【摘要】：从湖南省汨罗市血吸虫防治基地土壤中分离得到1株灭螺活性较强的微生物菌株B59,以B59菌株的总DNA为模板,采用细菌16S rDNA通用引物进行PCR扩增并测其核酸序列,得到1条约1 500 bp的片段。通过Blast软件在Gen Bank中进行同源性检索,将其与同源性较高的菌株的ITS序列进行Clustal X序列差异和同源性分析,分别使用MEGA 6.0软件中的邻接法、Phylip软件中的最大简约法及最大似然法构建系统发育树。结果显示,3种方法构建的系统发育树基本一致,B59菌株与Bacillus cereus的亲缘关系最近,聚为一支,相似度达100%。
[Abstract]:A microorganism strain B59 was isolated from the soil of Schistosoma japonicum control base in Miluo City, Hunan Province. Using the total DNA of strain B59 as template, the 16s rDNA primers were used for PCR amplification and nucleic acid sequencing. The fragment of 1 500 BP was obtained. The homology was searched in Gen Bank by Blast software, and the difference and homology of Clustal X sequence were analyzed with the ITS sequence of high homology strain. A phylogenetic tree was constructed by using the maximum minimization method and the maximum likelihood method in MEGA 6.0. The results showed that the phylogenetic tree constructed by the three methods was closely related to Bacillus cereus, and the similarity was 100%.
【作者单位】：长治医学院微生物教研室;中南大学湘雅医学院病原生物教研室;长沙金城医学检验所有限公司;
【基金】：国家自然科学基金(编号:81271862) 国家科技支撑计划(编号:2009BAI78B05)
【分类号】：R184.38

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