砷暴露对子代小鼠肺组织ERα表达性别差异的初步研究

发布时间：2018-04-26 21:01

本文选题：雌激素受体α + 肺组织　；参考：《四川大学学报(医学版)》2015年02期

【摘要】：目的观察亲代母鼠砷染毒后子代不同发育时期肺组织内雌激素受体α(ERα)的表达变化规律及性别间的差异,探讨砷暴露在肺癌发生性别差异中的作用。方法亲代母鼠在孕第8d至子代断乳期砷灌胃染毒,子代在断乳期后砷饮水染毒至成年期。通过实时荧光定量PCR和Western blot技术检测子代雌雄小鼠在不同发育时期、不同剂量(低、中、高)亚砷酸钠染毒情况下,肺组织中ERαmRNA和蛋白的表达水平。结果胚胎期雌性肺组织中ERαmRNA表达水平低于雄性(P0.05);断乳期和成年期雌性肺组织中ERαmRNA表达水平高于雄性(断乳期中剂量组P0.05,成年期中、高剂量组P0.05);胚胎期、断乳期、成年期各期对照组肺组织内ERαmRNA的表达水平差异无统计学意义;断乳、成年期雌性肺组织内ERαmRNA的表达水平均高于胚胎期雌性(低、中、高剂量组P0.05)。断乳期和成年期雌性各染毒剂量组肺组织中ERα蛋白表达水平均高于雄性,与对照组比较亦增高(P0.05)。成年期子代雌性、雄性小鼠各砷染毒组肺组织ERα蛋白的表达水平均高于断乳期(P0.05)。结论亲代母鼠砷染毒可使子代雌性小鼠在断乳期、成年期时肺组织内ERα表达水平均高于雄性小鼠,对阐明环境污染物在肺癌发生性别差异中的作用具有重要的意义。
[Abstract]:Objective to observe the changes of estrogen receptor 伪 er 伪 (ER 伪) expression in the lung tissues of maternal mice exposed to arsenic in different developmental stages, and to explore the role of arsenic exposure in the development of lung cancer. Methods the maternal mice were administrated with arsenic by stomach from the 8th day of gestation to the weaning stage of their offspring, and the offspring were exposed to arsenic in drinking water until adulthood after the weaning period. The expression levels of ER 伪 mRNA and protein in lung tissues of female and male offspring were detected by real-time fluorescence quantitative PCR and Western blot under different doses (low, medium and high) of sodium arsenite at different developmental stages. Results the expression level of ER 伪 mRNA in female lung tissue at embryonic stage was lower than that in male lung tissue, and the expression level of ER 伪 mRNA in female lung tissue during weaning and adulthood was higher than that in male group (P0.05 in middle dose group, P0.05 in high dose group in adult stage, P0.05 in embryo stage and weaning stage in adult stage). There was no significant difference in the expression of ER 伪 mRNA in lung tissue of adult control group, but the expression level of ER 伪 mRNA in female lung tissue of adult stage was higher than that of female group at embryonic stage (P 0.05) in low, middle and high dose groups. The expression of ER 伪 protein in lung tissues of female and adult groups was higher than that of male, and the expression of ER 伪 protein was also higher than that of control group (P 0.05). The expression level of ER 伪 protein in adult female and male mice was higher than that in brooding stage. Conclusion the expression of ER 伪 in the lung tissues of female mice was significantly higher than that of male mice during the weaning and adulthood, which was important to clarify the role of environmental pollutants in the development of lung cancer.
【作者单位】：四川大学华西公共卫生学院环境卫生与职业医学系;昆明市疾病预防控制中心;
【基金】：国家自然科学基金(No.81160340)资助
【分类号】：R114

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