增塑剂邻苯二甲酸二乙基己酯对昆明小鼠神经行为学的影响

发布时间：2018-04-29 07:27

本文选题：邻苯二甲酸二乙基己酯 + 学习　；参考：《华中师范大学》2013年硕士论文

【摘要】：邻苯二甲酸二乙基己酯(Di-(2-ethy lhexyl)phthalate, DEHP)作为一种人工合成有机化合物,以其良好的化学性质和低廉的成本被广泛用于塑料工业中,常被用作增塑剂添加到聚氯乙烯、聚乙稀、聚苯乙烯、聚丙烯塑料等塑料制品中,从而增加塑料制品的柔韧性和延展性、增强可塑性、提高塑料制品的强度。随着使用时间的推移,它很容易从塑料中释放,转移至外环境,扩散至空气、土壤和水体中,造成对环境、生物、食品的污染。DEHP可以通过经口、呼吸吸入、皮肤接触、医疗暴露等途径进入人体。对于一般人群,从食物中吸收成为DEHP进入人体的主要方式。DEHP具有多种生物毒性,包括遗传毒性、神经毒性、生殖毒性、免疫毒性和氧化损伤作用等。目前的研究项目中,对DEHP的生殖毒性的研究课题相对较多,而对神经毒性研究比较少。在本项研究的第一阶段实验中,选取雄性昆明小鼠作为研究对象,对其使用不同浓度的DEHP(0,5,50,500mg/kg)进行连续10天的灌胃染毒暴露,通过Morris水迷宫对其学习与记忆能力进行测试,并对脑组织中活性氧簇(Reactive oxygen species, ROS)和丙二醛(Malondialdehyde, MDA)含量进行检测,以判断DEHP所造成的脑组织氧化损伤程度。随后的第二阶段实验中,选用DEHP (50mg/kg)灌胃染毒同时添加维生素E (50mg/kg)作为保护剂,采用同样的方法对小鼠的学习与记忆能力进行检测,同时测定小鼠脑组织中ROS与MDA的含量,来判断维生素E对DEHP所产生的脑组织氧化损伤以及其引起的学习记忆能力减退的影响。第三阶段实验室关于小鼠情绪的研究,使用DEHP (500mg/kg)灌胃染毒暴露与氢化可的松阳性对照组相对比,分别通过开旷场实验、悬尾实验以及强迫游泳实验,构建抑郁模型,对小鼠的情绪以及抑郁程度进行测定。研究结果表明随着DEHP暴露水平的增加,小鼠脑组织中的活性氧(ROS)和丙二醛(MDA)含量随之增加,并且学习和记忆能力随之降低；添加维生素E作为保护剂的小鼠脑组织氧化损伤损伤程度,以及由此造成的的学习和记忆能力减退有缓解；同时DEHP可能会导致小鼠的抑郁情绪。而导致其学习记忆能力减退级产生抑郁情绪的机制可能是由于DEHP所诱导的小鼠脑组织氧化应激造成的。
[Abstract]:Diethylhexyl phthalate (DEHP), as a synthetic organic compound, is widely used in the plastics industry because of its good chemical properties and low cost, and is often used as a plasticizer for polyvinyl chloride (PVC), polystyrene (PS), polyvinyl chloride (PVC), polystyrene (PS), polyvinyl chloride (PVC), polyvinyl chloride (PVC) and polystyrene (PS). Polypropylene plastics and other plastic products, so as to increase the flexibility and ductility of plastic products, enhance plasticity, improve the strength of plastic products. And over time, it's very easy to release it from plastics, to move it out into the environment, to spread to the air, to the soil, to the water, to cause pollution to the environment, to living things, to food. DEHP can be inhaled through the mouth, breathed, and exposed to the skin. Medical exposure and other ways into the human body. For the general population, the main way to absorb DEHP from food into human body. DEHP has a variety of biological toxicity, including genotoxicity, neurotoxicity, reproductive toxicity, immune toxicity and oxidative damage and so on. Among the current research projects, there are more research topics on reproductive toxicity of DEHP, but less on neurotoxicity. In the first phase of this study, male Kunming mice were selected as the study subjects, and their learning and memory abilities were tested by Morris water maze. The contents of reactive oxygen specieses (Ross) and malondialdehyde malondialdehyde (MDAs) in brain tissue were determined to determine the degree of oxidative damage caused by DEHP. Then in the second phase of the experiment, the mice were given 50 mg / kg DEHP and 50 mg / kg vitamin E respectively as protective agent. The learning and memory abilities of mice were measured by the same method, and the contents of ROS and MDA in the brain tissue of mice were also measured. To determine the effects of vitamin E on brain oxidative damage and learning and memory impairment caused by DEHP. The emotion of mice was studied in the third stage of laboratory. The depression model was established by open field experiment, suspension test and forced swimming experiment, compared with hydrocortisone positive control group by intragastric administration of DEHP 500 mg / kg. The mood and depression of the mice were measured. The results showed that the contents of reactive oxygen species (Ros) and malondialdehyde (MDA) in brain tissue increased with the increase of DEHP exposure level, and the ability of learning and memory decreased. The degree of oxidative damage to brain tissue and the resulting impairment of learning and memory in mice treated with vitamin E as a protective agent were alleviated, and DEHP may lead to depression in mice. The mechanism of depression may be caused by oxidative stress in brain tissue induced by DEHP.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114

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