基于秀丽隐杆线虫的4种纳米材料的毒性研究

发布时间：2018-04-29 07:43

  本文选题：纳米材料 + 秀丽隐杆线虫　； 参考：《毒理学杂志》2015年06期

【摘要】：目的本研究对线虫暴露于金纳米团簇和纳米碳点及其偶联抗体产物(共4种医用纳米探针)后的感知行为和表型指标进行初步研究,以期探索应用线虫评价纳米材料安全性的新模式。方法将模式生物线虫用于金纳米团簇和纳米碳点的毒性研究,分别观察纳米材料对线虫的感知行为(趋避指数、趋向指数)、线虫表型指征(体型、灰度值、卷曲度、活动能力4个方面共16个指标)是否有改变,以判断其毒性作用大小。结果金纳米团簇(≤3.00 mg/ml)、偶联抗体金纳米团簇(≤3.00 mg/ml)、碳点(≤1.51 mg/ml)及偶联抗体碳点(≤3.50 mg/ml)趋避实验为阴性;在趋向试验中,3.00、1.40和0.30 mg/ml金纳米团簇、0.33 mg/ml碳点的趋向指数为阳性。16个非致死性指标总阳性率分别为偶联抗体碳点(81%)偶联抗体金纳米团簇(69%)金纳米团簇(56%)碳点(50%)。结论本研究初步探讨了4种纳米材料对线虫感知行为的影响,且将线虫的表型指征首次应用于纳米材料毒性研究中,为探索纳米材料的毒性提供了新颖的、有一定参考价值的快速筛选的指标。
[Abstract]:Objective to study the sensing behavior and phenotypic indexes of nematodes exposed to gold nanoclusters and carbon nanoparticles and their coupling antibody products (including four kinds of medical nanoprobes). In order to explore the application of nematodes to evaluate the safety of nanomaterials a new model. Methods the model biological nematode was used to study the toxicity of gold nanoclusters and carbon nanoparticles, and the perceived behavior of nanomaterials to nematodes (avoidance index, trend index, phenotypic indication of nematodes (shape, gray value, curvature) were observed, respectively. There were 16 indexes in 4 aspects of activity ability) to judge the toxicity. Results Gold nanoclusters (鈮，

本文编号：1819038