2012年广西壮族自治区一起成人流行性腮腺炎暴发的病原基因型特征

发布时间：2018-04-30 04:02

本文选题：流行性腮腺炎 + 暴发　；参考：《中国疫苗和免疫》2017年01期

【摘要】：目的了解2012年广西一起成人流行性腮腺炎(流腮)暴发的病原基因型特征,为制定防治流腮策略提供依据。方法将病例咽拭子接种到淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/SLAM)进行病毒分离,应用逆转录-聚合酶链反应(RT-PCR)扩增小疏水蛋白(SH)基因片段,进行测序和分析。结果从8个病例咽拭子标本中,分离到4株腮腺炎病毒,均属于F基因型,与国内外近年流行的其他F基因型腮腺炎病毒株核苷酸和氨基酸同源性分别为93.9%-99.6%和91.2%-100%,其中与2009年广西株核苷酸同源性和氨基酸同源性分别为95.8%-96.5%和92.9%;与中国疫苗株S79核苷酸差异高达14.3%。结论该疫情是由F基因型腮腺炎病毒引起的,与2009年广西株属于两个不同的传播链。应加强流腮的实验室监测,以及时有效地预防和控制流腮暴发。
[Abstract]:Objective to investigate the genotypic characteristics of adult mumps (gills) outbreak in Guangxi in 2012, and to provide evidence for formulating strategies for prevention and treatment of Mumps. Methods the throat swabs were inoculated into the kidney cells of African green monkeys transfected with lymphoid signal activator for virus isolation. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the small hydrophobic protein SHN gene fragment and sequenced and analyzed. Results four strains of mumps virus were isolated from throat swabs of 8 cases, all of which belonged to F genotype. The nucleotide and amino acid homology were 93.9-99.6% and 91.2-100 with other F genotypes mumps virus strains which were prevalent at home and abroad in recent years. The nucleotide homology and amino acid homology with Guangxi strain in 2009 were 95.8-96.5% and 92.9%, respectively, and those with Chinese vaccine strains were 95.8% -96.5% and 92.9%, respectively. The difference of S 79 nucleotides was as high as 14. 3%. Conclusion the epidemic was caused by the F genotype mumps virus and belonged to two different transmission chains as compared with Guangxi strain in 2009. Laboratory monitoring of gills should be strengthened in order to prevent and control the gills outbreak in time and effectively.
【作者单位】：广西壮族自治区疾病预防控制中心;
【分类号】：R512.1;R181.3

【相似文献】