N-氨基甲酰-L-谷氨酸对断奶小鼠生长性能的影响

发布时间：2018-05-03 18:14

本文选题：N-氨甲酰-L-谷氨酸 + 精氨酸　；参考：《南昌大学》2012年硕士论文

【摘要】：动物由于断奶应激会出现无食欲、免疫力下降、生长减缓等多种症状,研究证明断奶后由于饲料中精氨酸(Arg)不能满足动物生长的需要,是这些症状形成的重要原因之一。因此提高动物断奶后内源性精氨酸的合成可以有效减轻断奶应激反应,更好的促进断奶动物的生长。 N-氨甲酰-L-谷氨酸(N-Carbamyl-L-glutamic acid, NCG)是N-乙酰-L-谷氨酸(N-Acetyl-L-glutamic acid, NAG)的化学结构类似物。在动物体内,它能产生与NAG相同的生理功能——催化氨甲酰磷酸合成酶-I(CPS-I)的生成,进而促进谷氨酰胺、脯氨酸等转化为精氨酸,从而增加精氨酸的内源性合成,另外由于细胞内没有降解NCG的脱酰酶,NCG几乎不被分解,因而很小的剂量就能产生显著作用。因此,NCG对改善动物断奶后精氨酸合成及吸收量不能满足生长代谢需要而引起的各种症状会产生较好的效果。本文对NCG及其与Arg的联合作用对断奶小鼠生长性能、血清生化指标、血清精氨酸含量、免疫器官指数的影响进行了研究。主要研究内容与结果归纳如下： 1.研究了日粮中添加不同浓度NCG对断奶小鼠生长性能的影响。在断奶小鼠基础日粮中添加0.025%、0.05%、0.1%的NCG,通过监测小鼠体重的变化,平均日增重、日采食量、料重比等数据研究NCG对断奶小鼠生长性能的影响。实验结果发现日粮中添加0.1%的NCG,断奶小鼠平均日增质量与对照组相比提高了12.40%,饲料与体质量比与对照组相比下降了14.65%。结果表明日粮中添加适量浓度的NCG能显著提高断奶小鼠生长性能。 2.研究了NCG与Arg联合作用对断奶小鼠生长性能的影响。选择在基础日粮中同时添加0.05%的NCG和三个水平的Arg(0.025%、0.05%、0.1%),研究NCG与Arg联合作用对断奶小鼠生长性能的影响。实验结果发现同时添加0.05%的NCG和0.025%的Arg,断奶小鼠平均日增质量提高了10.53%。结果表明适量浓度NCG与Arg的联合作用,能显著提高断奶小鼠生长性能,并且提高生长性能的效果比单独添加NCG、Arg要好。同时还发现单独添加Arg只能在断奶后初始阶段对生长性能有较好的促进作用,之后效果不佳,而0.1%NCG组和低剂量Arg联合作用在整过实验阶段都有较好的促生长作用。 3.研究了NCG对断奶小鼠血清中谷丙转氨酶(GPT)、血清尿素氮(BUN)、总蛋白、白蛋白、球蛋白含量的影响。结果表明NCG及其与Arg联合作用对断奶小鼠血清GPT没有显著影响；高NCG组和高Arg联合组BUN值显著高于对照组,推测高浓度NCG及高浓度Arg与中剂量NCG联合作用可能对小鼠肾脏造成一定的损伤。高NCG组、NCG与Arg联合作用组与对照组相比总蛋白和白蛋白都没有显著差异,中NCG组总蛋白、白蛋白含量显著低于对照组,说明NCG促进生长性能的提高与血清中总蛋白、白蛋白的含量没有直接联系。 3.研究了NCG对断奶小鼠血清中游离Arg的质量浓度的影响。实验结果发现,在基础日粮中添加0.05%和0.1%的NCG,都能显著提高血清中游离Arg的质量浓度,与对照组相比分别提高了21.00%和42.77%,并且数据表明NCG引起机体内Arg合成量的变化并不与NCG的添加量成正比。综合平均日增质量和血清游离Arg含量数据发现,生长速率的提高与提高机体内Arg的转化量有关系但不成正比例,推测NCG在提高生长性能的贡献上除了提高内源性Arg合成量外还有其它的作用。 4.研究了NCG对断奶小鼠免疫器官指数的影响。实验发现NCG对断奶小鼠脾脏指数的影响有一定的线性关系,但作用不显著,对胸腺指数影响较小。NCG与Arg联合作用组断奶小鼠脾脏指数和胸腺指数与空白对照组相比都没有显著差异,表明NCG与Arg联合作用对断奶以后17天小鼠免疫器官指数没有显著性作用。
[Abstract]:It is one of the important reasons for the formation of these symptoms after weaning because of the inability of arginine (Arg) in feed to meet the needs of animal growth. Therefore, the improvement of endogenous arginine synthesis can effectively reduce weaning stress after weaning. The reaction is better to promote the growth of the weaned animals.
N- -L- glutamic acid (N-Carbamyl-L-glutamic acid, NCG) is a chemical structural analogue of N- acetyl -L- glutamic acid (N-Acetyl-L-glutamic acid, NAG). In animals, it can produce the same physiological function as NAG, catalyze the formation of aminoformyl phosphate synthetase -I, and then promote the transformation of glutamine, proline, and so on into arginine. Acid, thus increasing the endogenous synthesis of arginine, and in addition to the lack of dedeoylase of NCG in cells, NCG is almost not decomposed, so a very small dose can produce significant effects. Therefore, NCG has a good effect on the various symptoms caused by the improvement of the synthesis and absorption of arginine after weaning in animals that can not meet the needs of growth metabolites. The effects of the combination of NCG and Arg on the growth performance, serum biochemical indexes, serum arginine content and immune organ index of weaned mice were studied in this paper.
The main research contents and results are summarized as follows:
1. the effects of adding different concentrations of NCG on the growth performance of weaned mice were studied. Adding 0.025%, 0.05%, 0.1% NCG in the basal diet of weaned mice, the effects of NCG on the growth performance of weaned mice were studied by monitoring the changes of weight of mice, average daily weight gain, daily feed intake and feed weight ratio. The experimental results showed that the diet added 0 to the diet. .1% NCG, the average daily mass of weaning mice increased by 12.40% compared with the control group. The feed and body mass ratio decreased significantly compared with the control group. The addition of a proper concentration of NCG in the 14.65%. result table could significantly increase the growth performance of the weaned mice.
2. the effect of the combination of NCG and Arg on the growth performance of weaned mice was studied. The effects of the combination of 0.05% NCG and three levels of Arg (0.025%, 0.05%, 0.1%) on the basal diet were selected to study the effects of the combined action of NCG and Arg on the growth performance of weaned mice. The experimental results showed that 0.05% NCG and 0.025% Arg were added to the experiment, and the average day of weaning mice was found. The 10.53%. results showed that the combined effect of appropriate concentration of NCG and Arg could significantly improve the growth performance of weaned mice, and the effect of increasing growth performance was better than that of adding NCG alone and Arg. At the same time, it was found that the addition of Arg alone could have a better effect on Growth in the initial stage of weaning. After that, the effect was not good, and 0 The combined action of.1%NCG group and low dose Arg had a good growth promoting effect in the whole experimental stage.
3. the effects of NCG on alanine transaminase (GPT), serum urea nitrogen (BUN), total protein, albumin and globulin content in the serum of weaned mice were studied. The results showed that the combination of NCG and Arg had no significant effect on serum GPT in weaned mice, and the BUN value of high NCG group and high Arg group was significantly higher than that of the control group, and the high concentration NCG and high concentration Arg were presumed to be Arg. The combined action of the medium dose NCG could cause certain damage to the kidney of mice. In the high NCG group, there was no significant difference in total protein and albumin in the combination group of NCG and Arg compared with the control group. The content of total protein and albumin in the NCG group was significantly lower than that of the control group. It showed that the increase of the growth energy of the NCG and the content of the total protein and albumin in the serum There is no direct connection.
3. the effect of NCG on the mass concentration of free Arg in the serum of weaned mice was studied. The results showed that adding 0.05% and 0.1% NCG in the basal diet could significantly increase the concentration of free Arg in the serum, which was increased by 21% and 42.77% compared with the control group, and the data showed that the NCG caused the change of Arg synthesis in the body. It is directly proportional to the amount of NCG added. The comprehensive average daily mass and serum free Arg content data show that the increase of the growth rate is related to the increase of the transformation of Arg in the body, but it is not proportional. It is speculated that the contribution of NCG to the increase of growth performance is other than the increase of endogenous Arg synthesis.
4. the effect of NCG on the immune organ index of weanling mice was studied. It was found that NCG had a certain linear relationship with the spleen index of weaned mice, but the effect was not significant. There was no significant difference in spleen index and thymus index between.NCG and Arg in the group of weaning mice compared with that of the empty white control group, indicating that NCG Combined with Arg had no significant effect on immune organ index in 17 days after weaning.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

【参考文献】