饮食铁含量和长期运动对雄性大鼠外周及海马铁状态的影响

发布时间：2018-05-04 00:38

本文选题：运动 + 铁状态　；参考：《广州医学院》2012年硕士论文

【摘要】：研究目的通过给予不同铁含量饮食以及长期大强度游泳运动，观察雄性大鼠血液铁状态、肝脏非血红素铁(NHI）含量、海马NHI含量、海马脂质过氧化作用和羟自由基代谢的变化，探讨饮食铁含量和长期运动对雄性大鼠外周及海马铁状态的改变和意义。研究方法断乳雄性SD大鼠90只，分为饮食低铁含量组（12mg/kg），饮食标准铁含量组（45mg/kg）和饮食高铁含量组（1000mg/kg）。每组再分为运动组和静息组，即为六组，分别为低铁含量饮食+运动（EL）、低铁含量饮食+静息（SL）、标准铁含量饮食+运动（ES）、标准铁含量饮食+静息（SS）、高铁含量饮食+运动（EH）和高铁含量饮食+静息（SH）。各组用相应的铁含量饲料喂养1个月后，运动组开始游泳，每周5天，每天1次，，持续3个月，静息组除不进行运动外，其余处理同对应运动组。在最后一次运动后，大鼠空腹24h，在戊巴比妥钠麻醉下取静脉血，测定红细胞相关指标，包括血红蛋白（Hb）、红细胞压积（Hct）、平均红细胞体积（MCV）、平均红细胞血红蛋白量（MCH）、红细胞分布宽度（RDW）和血清铁状态指标，包括血清铁（SI）、血浆总铁结合力（TIBC）并计算转铁蛋白饱和度（TS）。取肝脏、海马组织液氮速冻，－80°C冰箱保存，测定肝脏、海马NHI，海马丙二醛含量（MDA）、抑制羟自由基（·OH）能力、超氧化物歧化酶活性（SOD）。研究结果 1.饮食铁含量和长期运动对雄性大鼠外周铁状态的影响饮食铁含量对Hb、Hct、MCV、MCH、RDW、SI、TIBC、TS和肝脏NHI均有影响（P0.05），运动对RDW、TIBC均有影响（P0.05）。与SS组比较，SL组Hb、Hct、MCV、MCH、SI、TS和肝脏NHI均降低（P0.05），TIBC及RDW均显著升高（P0.01）；SH组MCV降低（P0.05），肝脏NHI显著升高（P0.01），Hb、Hct、MCH、RDW、SI、TIBC和TS均无差异（P0.05）。与ES组比较，EL组Hb、Hct、MCV、MCH、SI、TS和肝脏NHI均降低（P0.05），TIBC及RDW均显著升高（P0.01）；EH组肝脏NHI显著升高（P0.01），Hb、Hct、MCV、MCH、RDW、SI、TIBC和TS均无差异（P0.05）。与SL组比较，EL组TS降低（P0.05），TIBC显著升高（P0.01），Hb、Hct、MCV、MCH、RDW、SI和肝脏NHI均无差异（P0.05）。与SS组比较，ES组RDW升高（P0.05），Hb、Hct、MCV、MCH、SI、TIBC、TS和肝脏NHI均无差异（P0.05）。与SH组比较，EH组肝脏NHI显著降低（P0.01），RDW升高（P0.05），Hb、Hct、MCV、MCH、SI、TIBC和TS均无差异（P0.05）。 2.饮食铁含量和长期运动对雄性大鼠海马铁状态、海马脂质过氧化作用、羟自由基代谢和SOD的影响饮食铁含量对海马抑制羟自由基能力及SOD均有影响（P0.05），运动对海马MDA及抑制羟自由基能力均有影响（P0.05）。与SS组比较，SL组海马MDA及抑制羟自由基能力均降低（P0.05），海马NHI及SOD均无差异（P0.05）；SH组海马抑制羟自由基能力升高（P0.05），海马NHI、MDA及SOD均无差异（P0.05）。与ES组比较，EL组海马NHI及抑制羟自由基能力均降低（P0.05），海马MDA及SOD均无差异（P0.05）；EH组海马抑制羟自由基能力及SOD均显著升高（P0.01），海马NHI及MDA均无差异（P0.05）与SL组比较，EL组海马NHI及抑制羟自由基能力均降低（P0.05），海马MDA显著升高（P0.01），海马SOD无差异（P0.05）与SS组比较，ES组海马NHI、MDA、抑制羟自由基能力及SOD均无差异（P0.05）。与SH组比较，EH组海马NHI、MDA、抑制羟自由基能力及SOD均无差异（P0.05）。研究结论 1.长期低铁含量饮食会导致大鼠营养性铁缺乏，肝脏贮存铁含量减少；大鼠高铁含量饮食时，Hb、血清铁等血液学指标均无变化，但肝脏内贮存铁增加，出现铁过载。 2.在低铁含量饮食时，长期运动会加剧大鼠血清铁状态的降低，而肝脏贮存铁含量无明显变化，提示肝脏具有维持最低水平铁贮存的机制；在铁含量饮食足够时，长期运动会导致RDW升高，肝脏NHI没有明显变化，但有降低的趋势；在高铁含量饮食时，长期运动会降低肝脏NHI含量，减轻肝脏铁负荷。 3.饮食铁含量与运动单独作用于雄性大鼠时，海马NHI含量均没有变化。在低铁含量饮食时，长期运动会加剧海马NHI的降低；在饮食铁含量充足及高铁含量饮食时，运动后海马NHI无显著变化。 4.长期低铁含量饮食会增加海马羟自由基水平，造成对海马组织的自由基损伤；高铁含量饮食时，海马羟自由基水平明显降低、SOD活力明显升高，说明适度增大铁含量的摄入有利于减少海马组织的自由基损伤。在低铁含量饮食时，长期运动会升高海马的羟自由基水平以及脂质过氧化，加剧对海马组织的过氧化损伤；在铁含量饮食足够或高铁含量饮食时，长期运动后海马NHI、脂质过氧化、羟自由基水平以及SOD均没有变化。
[Abstract]:research objective
The iron status of the male rats, the content of the liver non heme iron (NHI), the NHI content of the hippocampus, the lipid peroxidation and the hydroxyl free radical metabolism in the hippocampus were observed by giving different iron content diet and long term large intensity swimming exercise. The changes and meaning of iron content and long-term movement of the diet on the peripheral and hippocampal iron status of the male rats were discussed. Righteousness.
research method
90 male SD rats were divided into diet low iron content group (12mg/kg), dietary standard iron content group (45mg/kg) and diet high iron content group (1000mg/kg). Each group was divided into exercise group and resting group, that is, six groups were low iron content diet + exercise (EL), low iron content diet + resting (SL), standard iron content diet + exercise (ES), and standard iron content. Diet + resting (SS), high iron content diet + exercise (EH) and high iron content diet + resting (SH). Each group was fed with the corresponding iron content feed for 1 months, the exercise group began to swim, 5 days a week, 1 times a day, lasting 3 months, rest in the rest of the same exercise group. After the last exercise, the rat fasting 24h, at amyl Venous blood was taken under barbiturate sodium anesthesia to determine red blood cell related indicators, including hemoglobin (Hb), red blood cell pressure (Hct), mean red cell volume (MCV), average red blood cell hemoglobin (MCH), red cell distribution width (RDW) and serum iron status, including serum iron (SI), plasma total iron junction (TIBC) and transferrin saturation (T) (T). S). Liver, hippocampal liquid nitrogen quick freezing, - 80 degree C refrigerator was preserved, liver, hippocampal NHI, hippocampal malondialdehyde content (MDA), hydroxyl free radical (. OH) ability and superoxide dismutase activity (SOD) were suppressed.
Research results
1. effects of dietary iron content and long-term exercise on peripheral iron status in male rats
Dietary iron content has effects on Hb, Hct, MCV, MCH, RDW, SI, TIBC, TS and liver NHI (P0.05). Exercise has an effect on RDW and NHI.
Compared with the SS group, SL group Hb, Hct, MCV, MCH, SI, TS and liver NHI all decreased (P0.05), TIBC and RDW were significantly higher.
Compared with the ES group, EL group Hb, Hct, MCV, MCH, SI, TS and liver NHI all decreased (P0.05), TIBC and RDW all increased significantly.
Compared with group SL, TS in group EL decreased (P0.05), TIBC increased significantly (P0.01), Hb, Hct, MCV, MCH, RDW, liver and liver had no difference.
Compared with group SS, RDW in group ES increased (P0.05), Hb, Hct, MCV, MCH, SI, TIBC, TS and liver were no difference.
Compared with group SH, the liver NHI of group EH was significantly decreased (P0.01), RDW increased (P0.05), Hb, Hct, MCV, MCH, SI, and MCV had no difference.
2. effects of dietary iron content and long-term exercise on hippocampal iron status, hippocampal lipid peroxidation, hydroxyl free Kidese and SOD in male rats
Iron content in diet affected hippocampal inhibition of hydroxyl free radical and SOD (P0.05). Exercise had an effect on hippocampal MDA and inhibition of hydroxyl radical (P0.05).
Compared with group SS, hippocampal MDA and hydroxyl free radical inhibition in SL group were decreased (P0.05), NHI and SOD in hippocampus were not different (P0.05), and hippocampal inhibition of hydroxyl radical ability in SH group (P0.05), hippocampus NHI, MDA and SOD were all no difference.
Compared with the ES group, the hippocampal NHI and the inhibition of hydroxyl radical in EL group were decreased (P0.05), MDA and SOD in hippocampus were not different (P0.05). The hippocampal hippocampal inhibition of hydroxyl radical and SOD increased significantly in EH group (P0.01), and there was no difference in hippocampal NHI and MDA.
Compared with group SL, the hippocampal NHI and hydroxyl free radical were decreased in group EL (P0.05), MDA in hippocampus increased significantly (P0.01), and there was no difference in hippocampus SOD (P0.05).
Compared with group SS, there was no difference in hippocampal NHI, MDA, hydroxyl free radical and SOD in group ES (P0.05).
Compared with group SH, there was no difference in hippocampal NHI, MDA, hydroxyl free radical and SOD in group EH (P0.05).
research conclusion
1. long term low iron content diet could lead to nutritional iron deficiency in rats and reduced iron content in liver. No changes in hematological indexes such as Hb and serum iron were found in rats with high iron content diet, but iron accumulation in liver was increased and iron overload appeared in the liver.
2. in the diet of low iron content, long-term exercise would aggravate the reduction of iron status in the serum of rats, but there was no obvious change in the liver storage iron content, suggesting that the liver has the mechanism to maintain the lowest level of iron storage. When the iron content is sufficient, the long-term exercise will lead to the increase of RDW, and the liver NHI has no obvious change, but there is a decreasing trend; in Gao Tiehan Long term exercise reduces liver NHI content and reduces liver iron burden.
3. the content of iron in diet and exercise alone in the male rats had no change in the hippocampal NHI content. In the low iron diet, long term exercise aggravated the decrease of NHI in the hippocampus, and there was no significant change in the sports Houhai horse NHI in the diet iron content and the high iron content diet.
4. long term low iron content diet increases hippocampal hydroxyl radical level and causes free radical damage to hippocampal tissue. When high iron content diet, the level of hippocampal hydroxyl radical is significantly reduced and SOD activity is significantly increased. It is suggested that the moderate increase in iron intake is beneficial to reduce the free radical damage of hippocampus tissue. It increased the hydroxyl radical level of the hippocampus and lipid peroxidation, aggravated the oxidative damage to the hippocampus, and had no changes in the long-term exercise of Houhai horse NHI, lipid peroxidation, hydroxyl radical level and SOD when the iron content diet was sufficient or high iron content diet.

【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114

【参考文献】