徐州地区畜禽产品中沙门菌的监测及分子分型研究

发布时间：2018-05-04 01:06

本文选题：沙门菌 + 畜禽产品　；参考：《江苏师范大学》2017年硕士论文

【摘要】：沙门菌是全球范围内普遍存在的人畜共患食源性病原菌,其所导致的食源性疾病给人和动物的健康带来极大的危害。肉类食品因含有丰富的营养,为沙门菌的生长创造了良好条件,成为食源性沙门菌生长繁殖的主要场所,因此肉类食品中沙门菌的污染对于食品卫生状况和公共卫生安全问题有着重要的影响。本研究旨在了解徐州地区畜禽产品中沙门菌的流行状况、血清型分布特点,以及沙门菌的耐药情况,利用MLST、PFGE分子分型技术对它们进行基因层面的研究,从而在实验室基础上为制定沙门菌的防治策略和抗生素在畜禽生产中的合理使用提供科学依据,并找出不同沙门菌的内在联系以及沙门菌的潜在传播途径,从而在实验室基础上为食源性沙门菌的防控提供数据支持。一、徐州地区畜禽产品中沙门菌的分离鉴定及耐药性研究2015年5月至2016年10月,从徐州市多个农贸市场、连锁超市和养殖场共采集肉制品324份,共分离出沙门菌115株,总分离率为35.5%;血清型鉴定结果显示,115株沙门菌分离株中,鉴定出13种血清型,其中德尔卑沙门菌33株,分离率为28.7%,是徐州地区的优势血清型;对115株沙门菌进行16种抗生素敏感性测定,结果显示,115株沙门菌分离株中有107株沙门菌对四环素表现耐药,耐药率高达92%;而对β-内酰胺类抗生素表现出较高的敏感性,其中对美罗培南100%敏感。此外,沙门菌的多重耐药情况也较为严重,80%的菌株至少对3种抗生素表现耐药。二、徐州地区畜禽产品中沙门菌及人源沙门菌的分子分型研究利用多位点序列分型方法(MLST)将徐州地区不同来源的115株沙门菌分离株分成了 14种ST型,其中优势ST型为ST40,其次为ST11、ST463、ST469。脉冲场凝胶电泳(PFGE)将21株ST40型沙门菌和4株人源ST40型沙门菌分成了 19种PFGE型。4株人源沙门菌之间的相似度超过90%,与动物源沙门菌的相似度为59%,说明沙门菌可能在传播过程中随着宿主的改变而发生遗传物质的改。
[Abstract]:Salmonella is a worldwide common food borne pathogen of zoonotic, which causes the food borne diseases to bring great harm to the health of human and animal. Meat food has created good conditions for the growth of Salmonella because of its rich nutrition. It has become the main place for the growth and reproduction of food borne Salmonella, and therefore meat food The contamination of Salmonella spp. has an important impact on food hygiene and public health safety. This study aims to understand the epidemic situation of Salmonella in the livestock and poultry products in Xuzhou, the distribution characteristics of serotypes, and the drug resistance of Salmonella, and use MLST and PFGE molecular typing technology to study them at gene level. On the basis of the laboratory, a scientific basis for the prevention and control of Salmonella and the rational use of antibiotics in livestock and poultry production is provided, and the internal relations of different Salmonella and the potential transmission routes of Salmonella are found, and the data support for the prevention and control of the food borne Salmonella is supported on the basis of the laboratory. One, the livestock and poultry products in Xuzhou area Isolation, identification and drug resistance of Salmonella from May 2015 to October 2016, 324 parts of meat products were collected from several farm trade markets, chain supermarkets and farms in Xuzhou. 115 Salmonella strains were isolated and the total separation rate was 35.5%. The results of serotype identification showed that 13 serotypes were identified in 115 Salmonella isolates, including Del humpha. 33 strains of bacteria, with a separation rate of 28.7%, were the dominant serotypes in Xuzhou area, and 16 antibiotic susceptibility tests for 115 Salmonella strains. The results showed that 107 Salmonella isolates from 115 strains of Salmonella were resistant to tetracycline, and the resistance rate was 92%, while beta lactam was highly sensitive, including meropenem 100. In addition, the multiple drug resistance of Salmonella was also serious, and 80% of the strains were resistant to at least 3 kinds of antibiotics. Two, the molecular typing of Salmonella and Salmonella in livestock and poultry in Xuzhou area was divided into 14 ST isolates from 115 strains of Salmonella isolated from the same source in the Xuzhou region. The dominant ST type was ST40, followed by ST11, ST463, and ST469. pulse field gel electrophoresis (PFGE), which divided 21 strains of ST40 Salmonella and 4 strains of human ST40 Salmonella into 19 PFGE.4 strains of Salmonella. The similarity between Salmonella and Salmonella was 59%, indicating that Salmonella may be in the course of transmission with the host. Changes in genetic material change.

【学位授予单位】：江苏师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R155.5

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