镉对去卵巢大鼠肾脏的类雌激素作用

发布时间：2018-05-04 07:22

本文选题：镉 + 雌激素受体　；参考：《环境与健康杂志》2015年02期

【摘要】：目的观察镉染毒对去卵巢大鼠肾脏中雌激素受体α(ERα)、雌激素受体β(ERβ)表达的影响。方法将24只健康12周龄SPF级SD雌性大鼠随机分为3组,分别为假手术(蒸馏水)组、去卵巢(蒸馏水)组、染镉(200 mg/L)去卵巢组,每组8只。假手术组切除小块卵巢周围的脂肪,去卵巢组和染镉去卵巢组切除双侧卵巢。术后1周,采用自由饮水方式进行染毒,连续染毒12周。采用电感耦合等离子体质谱法测定大鼠血镉、尿镉水平,采用酶联免疫吸附测定血清雌二醇、β2-微球蛋白水平,采用Western blot技术检测肾脏组织中ERα、ERβ蛋白的表达水平。结果与假手术组相比,去卵巢组染镉组12周末体重下降(P0.05)、肾脏系数升高(P0.05),去卵巢组及染镉去卵巢组雌激素水平降低(P0.05);与去卵巢组相比,染镉去卵巢组体重、雌激素水平降低(P0.05),肾脏系数、β2-微球蛋白、尿镉及血镉水平升高(P0.05);染镉去卵巢组血镉与尿镉、β2-微球蛋白、肾脏ERβ的表达水平正相关(P0.05),而与ERα无统计学相关性(P0.05)。结论重金属镉可降低去卵巢大鼠血清雌激素水平及增加雌激素受体β受体表达,这可能是镉的肾毒性作用机制之一。
[Abstract]:Objective to observe the effect of cadmium exposure on the expression of estrogen receptor 伪 -ER 伪 and estrogen receptor 尾 -ER 尾 in the kidney of ovariectomized rats. Methods Twenty-four healthy 12-week-old SPF female rats were randomly divided into three groups: sham-operated (distilled water) group, ovariectomized (distilled water) group and ovariectomized group (200mg / L). In sham-operated group, fat around small ovary was removed, ovariectomized group and cadmium stained ovariectomized group. One week after operation, free drinking water was used for 12 weeks. Serum cadmium and urine cadmium levels were measured by inductively coupled plasma mass spectrometry (ICP-MS), serum estradiol and 尾 _ 2-microglobulin were measured by enzyme-linked immunosorbent assay (Elisa), and ER 伪 -ER 尾 protein expression in renal tissue was detected by Western blot technique. Results compared with sham-operation group, the weight of ovariectomized group decreased P0.05, the kidney coefficient increased P0.05, the estrogen level of ovariectomized group and cadmium-exposed ovariectomized group decreased P0.05, compared with ovariectomized group, the body weight of cadmium-exposed ovariectomized group was lower than that of ovariectomized group. Estrogen level decreased P0.05G, renal coefficient, 尾 2-microglobulin, urine cadmium and blood cadmium level increased P0.050.The expression level of blood cadmium and urine cadmium, 尾 2-microglobulin and renal ER 尾 was positively correlated with the expression level of ER 尾 in ovariectomized group (P 0.05), but not correlated with ER 伪 (P 0.05). Conclusion cadmium can decrease the serum estrogen level and increase the expression of estrogen receptor 尾 receptor in ovariectomized rats, which may be one of the mechanisms of renal toxicity of cadmium.
【作者单位】：中南大学地球科学与信息物理学院;桂林医学院附属医院肾内科;
【基金】：广西医疗卫生重点科研课题(桂重2012011) 桂林市科学研究与技术开发计划(20120121-1-3)
【分类号】：R114

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