甲醛对大鼠胚胎心脏的毒性研究

发布时间：2018-05-05 05:06

本文选题：甲醛 + 胚胎　；参考：《延边大学》2014年硕士论文

【摘要】：目的：本实验旨在研究甲醛对孕鼠胚胎心脏发育的毒性作用。 .方法：选择成功受孕的成年健康SD大鼠随机分四组,每组5只分别吸入不同浓度甲醛：第1组(对照组)：吸入新鲜空气,甲醛浓度接近于0mg/m3、第2组(低浓度组)：(4.8±1.3)mg/m3、第3组(中浓度组)：(25.24±4.6)mg/m3、第4组(高浓度组)：(119.64±15.4)mg/m3。各组大鼠均从孕第7天开始每日上午9：00吸入不同浓度甲醛进行染毒,每日2小时,连续染毒12天,建立染毒模型。待各组孕鼠自然分娩后,进行新生小鼠胚胎发育的一般毒性、心脏毒性检测,同时观察心脏的病理学改变。结果： 1、新生小鼠胚胎发育的一般毒性：四组新生小鼠平均出生体重分别为7.96g、7.88g、7.80g、7.01g。高浓度组分别与其它组两两比较,P值均0.05。 2、新生小鼠心脏发育的毒性：四组新生小鼠发生心脏畸形率分别为：0%、0%、0%、8.9%。高浓度组致小鼠畸形率明显高于其它组。 3、血清天门冬氨酸氨基转移酶水平：四组新生小鼠平均AST值分别为162.1U/L、170.0UL、207.3U/L和318.7U/L。各组间两两相比较,高浓度组、中浓度组小鼠血清中平均天门冬氨酸氨基转移酶水平明显高于低浓度组及正常对照组。 4、心脏组织超微结构改变：正常对照组心肌组织电镜下超微结构基本正常,高浓度组可见心肌组织肌原纤维排列紊乱；线粒体肿大,大小不一,嵴断裂、溶解,有较多空泡形成；线粒体间糖原颗粒少。 5、心脏组织HE染色：正常对照组心肌细胞正常,可见横纹,无变性坏死；甲醛低浓度组和中浓度组与正常组类似,但可见少量淋巴细胞侵润；甲醛高浓度组心脏有明显水肿变性,细胞胞浆内有颗粒样物质,个别细胞可见核浓缩、核碎裂等细胞坏死表现,心肌细胞横纹不清。结论1、甲醛具有胚胎毒性,可降低新生小鼠出生体重。 2、甲醛可导致新生小鼠心肌细胞发生变性坏死,影响心肌细胞正常功能。 3、甲醛对小鼠胚胎心脏可产生毒性作用。
[Abstract]:Objective: to study the toxic effect of formaldehyde on embryonic heart development in pregnant rats. . Methods: healthy adult SD rats who were successfully conceived were randomly divided into four groups with 5 rats in each group inhaling different concentrations of formaldehyde: group 1 (control group: inhalation of fresh air) The concentration of formaldehyde was close to 0 mg / m 3, group 2 (low concentration group: 4.8 卤1.3 mg / m 3), group 3 (middle concentration group: 25.24 卤4.6 mg / m 3), group 4 (high concentration group: 119.64 卤15.4mg / m 3). The rats in each group were exposed to different concentrations of formaldehyde at 9:00 every day from the 7th day of pregnancy for 2 hours daily for 12 consecutive days to establish the model of exposure. The common toxicity and cardiac toxicity of embryonic development of newborn mice were detected after the natural delivery of pregnant mice in each group, and the pathological changes of heart were observed at the same time. Results: 1. The general toxicity of embryonic development in newborn mice: the average birth weight of newborn mice in the four groups was 7.96g / 7.88g / 7.80g / 7.01grespectively. Compared with other groups, the P value of high concentration group was 0.05. 2. The toxicity of cardiac development in newborn mice: the incidence of cardiac malformation in the four groups of newborn mice was respectively 1: 0 and 8. 9%. The deformity rate of mice in high concentration group was significantly higher than that in other groups. 3, serum aspartate aminotransferase level: the average AST values of the four groups of newborn mice were 162.1 U / L 170.0ULN 207.3 U / L and 318.7 U / L, respectively. The average aspartate aminotransferase level in the serum of the mice in the high concentration group and the middle concentration group was significantly higher than that in the low concentration group and the normal control group. (4) the ultrastructural changes of cardiac tissue: the ultrastructure of myocardial tissue in the normal control group was basically normal under electron microscope, and in the high concentration group, the myofibril arrangement of myocardial tissue was disordered, the mitochondria was enlarged and varied in size, the crest was broken and dissolved, There were more vacuoles and less glycogen granules between mitochondria. 5, HE staining of heart tissue: normal control group showed normal myocardial cells with striation and no degeneration and necrosis, low and middle concentration groups of formaldehyde were similar to normal group, but a small amount of lymphocytes were infiltrated. In the high concentration group of formaldehyde, there were obvious edema and degeneration in the heart, granule like substance in the cytoplasm of the cells, nuclear concentration, nuclear fragmentation and other cell necrosis manifestations in individual cells, and the cross lines of the cardiac myocytes were unclear. Conclusion 1. Formaldehyde has embryotoxicity and can reduce birth weight of newborn mice. 2, formaldehyde can cause degeneration and necrosis of neonatal mouse cardiomyocytes and affect the normal function of cardiomyocytes. 3, formaldehyde can produce toxic effect on mouse embryonic heart.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R114

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