对氨基水杨酸钠对锰中毒大鼠学习记忆和神经发生的影响

发布时间：2018-05-07 20:30

本文选题：锰 + 对氨基水杨酸钠　；参考：《广西医科大学》2013年硕士论文

【摘要】：[目的]观察对氨基水杨酸钠(PAS-Na)对锰中毒大鼠学习记忆能力和神经发生水平的影响。[方法]40只健康成年雄性SD大鼠被随机分为对照组和染锰组。染锰组、每日腹腔注射(ip) MnCl2·4H2O15mg/kg,对照组ip等容量生理盐水,每周5天,连续6周。然后,将染锰组分为染锰组、低、中、高PAS-Na (L、M、H-PAS)治疗组,L、M、H-PAS治疗组每日分别背部皮下注射(sc) PSA-Na100、200或300mg/kg,对照组、染锰组背部sc等容量生理盐水,每周用药4天,停药3天,连续4周。采用Morris水迷宫系统检测大鼠学习记忆能力,免疫组化检测5-溴脱氧尿嘧啶核苷(BrdU)标记的神经发生细胞数量。[结果]染锰组逃避潜伏期、游泳路程均比对照组长,平台穿越次数比对照组少(P0.01)。各PAS-Na治疗组逃避潜伏期、游泳路程均较染锰组短,M-、H-PAS治疗组平台穿越次数比染锰组多(P0.05或0.01)。染锰组海马BrdU阳性细胞数目低于对照组,M-、H-PAS治疗组BrdU阳性细胞数目高于染锰组(P0.01)。[结论]锰暴露能减弱成年大鼠学习记忆能力,降低海马神经发生水平。PAS-Na可以改善锰中毒大鼠的学习记忆能力,促进其海马神经发生
[Abstract]:[objective] to observe the effects of sodium p aminosalicylate PAS-Naon on learning and memory ability and neurogenesis in rats with manganese poisoning. [methods] Forty healthy adult male SD rats were randomly divided into control group and manganese-exposed group. In the manganese exposed group, MnCl2 4H 2O 15 mg / kg daily was injected intraperitoneally, while the control group was given IP saline for 5 days a week for 6 weeks. Then, the manganese exposed group was divided into two groups: low, medium and high PAS-Na group. The treatment group was given subcutaneous injection of PSA-Na100200 or 300mg / kg PSA-Na100200 respectively. The control group and the manganese exposed group received saline of the same volume, 4 days a week, 3 days a week, and 4 weeks in succession. The learning and memory abilities of rats were measured by Morris water maze system and the number of neurogenesis cells labeled with 5-bromodeoxyuridine (BrdU) was detected by immunohistochemistry. [results] the escape latency and swimming distance of manganese exposed group were all higher than that of control group, and the times of platform crossing were less than that of control group (P 0.01). The escape latency and swimming distance of each PAS-Na treatment group were shorter than those of the manganese exposed group. The number of platform crossing in the treatment group was more than that in the manganese exposed group (P0.05 or 0.01). The number of BrdU positive cells in hippocampus of manganese exposed group was lower than that of control group. The number of BrdU positive cells in treatment group was higher than that in manganese exposed group. [conclusion] Manganese exposure can attenuate the learning and memory ability of adult rats and decrease the level of hippocampal neurogenesis. PAS-Na can improve the learning and memory ability and promote the development of hippocampal nerve in rats with manganese poisoning.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114

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