益生菌通过共轭亚油酸调节小鼠免疫功能

发布时间：2018-05-07 19:34

本文选题：共轭亚油酸(CLA) + 益生菌　；参考：《营养学报》2015年01期

【摘要】：目的研究共轭亚油酸(CLA)益生菌嗜酸乳杆菌F0221对小鼠免疫调节能力的影响。方法 30只8w龄雄性昆种小鼠随机分为:空白对照、F0221(阴性对照I)、亚油酸(阴性对照II)、低剂量F0221+LA、高剂量F0221+LA5组。喂养4 w后,处死。采用气相色谱-质谱测定回肠和结肠内容物中c9,t11 CLA含量,利用ELISA试剂盒测定血清白介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)和前列腺素E2(PGE2)浓度,计算脾脏和胸腺指数,并测定脾淋巴细胞增殖能力和腹腔巨噬细胞吞噬能力。结果与空白对照组相比,高剂量F0221+LA组小鼠回肠和结肠内容物中c9,t11 CLA含量、血清IL-2、TNF-α和PGE2浓度、脾淋巴细胞增殖能力和腹腔巨噬细胞吞噬能力均显著提高,但两剂量组之间上述指标无显著性差异。结论嗜酸乳杆菌F0221在小鼠体内利用肠道中的亚油酸合成c9,t11 CLA,进而显著增强机体的特异性和非特异免疫能力,其调节机制可能是通过降低PGE2体内合成能力实现的。
[Abstract]:Objective to study the effect of probiotic Lactobacillus acidophilus F0221 on immune regulation in mice. Methods Thirty 8-week-old male Kunming mice were randomly divided into three groups: blank control group F0221 (negative control), linoleic acid (negative control), low dose of F0221a, high dose of F0221 LA5. After 4 weeks of feeding, the rats were killed. The content of c9t11 CLA in ileum and colon was determined by gas chromatography-mass spectrometry (GC-MS), and the concentrations of serum interleukin-2 (IL-2), tumor necrosis factor- 伪 (TNF- 伪) and prostaglandin E _ 2 (PGE _ 2) were determined by ELISA kit. The index of spleen and thymus were calculated. The proliferation of splenic lymphocytes and phagocytosis of peritoneal macrophages were measured. Results compared with the control group, the content of c9t11 CLA in ileum and colon, the concentration of IL-2TNF- 伪 and PGE2, the proliferation ability of spleen lymphocytes and the phagocytosis of peritoneal macrophages were significantly increased in high dose F0221LA group. But there was no significant difference in the above indexes between the two dose groups. Conclusion Lactobacillus acidophilus F0221 synthesizes c9t11CLAs by using linoleic acid in the intestinal tract of mice, which can significantly enhance the specific and non-specific immune ability of the body, and its regulatory mechanism may be achieved by reducing the biosynthesis ability of PGE2 in vivo.
【作者单位】：西北农林科技大学食品科学与工程学院;
【基金】：国家自然科学基金-青年科学基金项目(No.31301444) 中央高校基本科研业务费专项资金(No.QN2013059)
【分类号】：R151

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