虾青素对铜离子诱导的前列腺细胞氧化损伤的影响（英文）

发布时间：2018-05-08 05:05

本文选题：氧化损伤 + PC-细胞　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年02期

【摘要】：目的:研究虾青素对铜离子诱导的前列腺细胞氧化损伤的影响,并探索其作用机制。创新点:首次研究虾青素对铜离子诱导的前列腺细胞及前列腺癌细胞氧化损伤的影响,并比较其对两种细胞作用的差异。方法:MTT法测定铜离子与虾青素对前列腺细胞(RWPE-1)和前列腺癌细胞(PC-3)生长的影响;采用细胞流式仪测定虾青素对铜离子诱导的RWPE-1和PC-3细胞凋亡的影响;荧光分光光度法测定了虾青素对铜离子诱导的活性氧自由基(ROS)产生的影响;采用罗丹明123(Rh123)染色检测虾青素对铜离子诱导的细胞线粒体膜电位(MMP)变化的影响;采用试剂盒测定了虾青素对铜离子存在下丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性变化的影响。结论:结果表明,铜离子能诱导RWPE-1和PC-3细胞凋亡,并伴随细胞内ROS和MDA含量升高;虾青素处理可显著降低RWPE-1细胞中MDA含量,升高线粒体膜电位,并保持ROS含量稳定;虾青素处理可降低PC-3细胞中SOD、GSH-Px和CAT的活性,而对RWPE-1细胞则作用相反。因此,虾青素处理能有效降低铜离子对RWPE-1细胞引起的损伤,而通过降低抗氧化酶活性加剧铜离子对PC-3细胞的损伤。
[Abstract]:Objective: To study the effect of astaxanthin on the oxidative damage of prostatic cells induced by copper ion and explore its mechanism. Innovation: the first study on the effects of astaxanthin on the oxidative damage of prostate cells and prostate cancer cells induced by copper ion, and comparing the difference between the two kinds of cells. Method: the determination of copper ions and astaxanthin by MTT method The effects on the growth of prostate cells (RWPE-1) and prostate cancer cells (PC-3); the effects of astaxanthin on the apoptosis of RWPE-1 and PC-3 cells induced by copper ions were measured by flow cytometry; the effect of astaxanthin on the production of ROS by copper ion induced by copper ion was measured by fluorescence spectrophotometry; and Luo Danming 123 (Rh123) staining was used to detect the effect of astaxanthin on the production of oxygen free radicals. The effect of astaxanthin on the changes of mitochondrial membrane potential (MMP) induced by copper ion; the effect of astaxanthin on the content of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity in the presence of copper ions in the presence of copper ions. Conclusion: the results show that copper ions can induce RWPE The apoptosis of -1 and PC-3 cells and the increase of intracellular ROS and MDA content, astaxanthin treatment can significantly reduce the MDA content in RWPE-1 cells, increase the mitochondrial membrane potential and keep the ROS content stable. Astaxanthin treatment can reduce SOD, GSH-Px and CAT activity in PC-3 cells, but to the RWPE-1 cells the opposite. Therefore, astaxanthin treatment can be effectively reduced. Low copper ions can cause damage to RWPE-1 cells, but reduce the damage of copper ions to PC-3 cells by reducing antioxidant enzyme activities.

【作者单位】： Department
【基金】：Project supported by the Plan of Medicine and Health Science and Technology of the Zhejiang Province of China(No.2012RCA022)
【分类号】：R151

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