生长发育期氯化镧暴露对大鼠海马紧密连接蛋白表达影响

发布时间：2018-05-11 14:06

本文选题：氯化镧 + 海马　；参考：《中国公共卫生》2017年07期

【摘要】：目的探讨氯化镧(LaCl_3)暴露对仔鼠海马紧密连接蛋白表达和基因转录水平影响。方法将40只Wistar孕鼠随机分为对照组(蒸馏水)和0.25%、0.50%、1.00%(w/v)氯化镧组,从仔鼠出生起孕鼠通过饮水染毒LaCl_3,断乳后仔鼠继续饮用原浓度LaCl_3溶液1个月;采用Western blot法检测仔鼠海马claudin-5、occludin和紧密连接蛋白-1(ZO-1)蛋白表达,real-time PCR法检测仔鼠海马Cldn5、Ocln和Tjp1 mRNA表达。结果与对照组比较,0.50%和1.00%氯化镧组仔鼠海马claudin-5蛋白表达水平[分别为(0.059±0.007)、(0.041±0.003)]与occludin蛋白表达水平[分别为(0.085±0.004)、(0.067±0.004)]均明显降低(P0.05);1.00%氯化镧组仔鼠海马Cldn5、Ocln和Tjp1 mRNA表达水平分别为(0.743±0.026)、(0.900±0.119)、(0.931±0.100),与对照组比较,1.00%氯化镧组仔鼠海马Cldn5 mRNA表达下降(P0.05)。结论生长发育期暴露氯化镧可下调仔鼠海马claudin-5和occludin表达,导致血脑屏障紧密连接受损。
[Abstract]:Objective to investigate the effects of LaCl3) exposure on the expression of connexin and gene transcription in hippocampus of neonatal rats. Methods Forty pregnant Wistar rats were randomly divided into two groups: control group (distilled water) and 0.25g / 0.50g / w / v) lanthanum chloride group. The pregnant mice were exposed to LACl3 through drinking water from the birth of their offspring, and the mice continued to drink the original LaCl_3 solution for 1 month after weaning. The expression of claudin-5occludin and tight junction protein (-1) ZO-1 in hippocampus of neonatal rats was detected by Western blot method. The expression of Tjp1 mRNA and Cldn5Ocln in hippocampus of neonatal rats were detected by real-time PCR method. Results compared with the control group, 0.50% and 1.00% lanthanum chloride groups significantly decreased the expression levels of claudin-5 protein and occludin protein in hippocampus of neonatal rats [0.059 卤0.007 卤0.003] and occludin protein (0.085 卤0.004 卤0.067 卤0.004), respectively. The expression levels of Cldn5Ocln and Tjp1 mRNA in hippocampus of neonatal rats in lanthanum chloride group were 0.743 卤0.0260.900 卤0.1919 卤0.100, respectively. Compared with the control group, the expression of Cldn5 mRNA in hippocampus of 1. 00% lanthanum chloride group decreased (P 0. 05). Conclusion exposure to lanthanum chloride during growth and development can down-regulate the expression of claudin-5 and occludin in hippocampus of neonatal rats, resulting in damage of tight junction of blood-brain barrier.
【作者单位】：锦州医科大学公共卫生学院职业卫生与环境卫生学教研室;中国医科大学公共卫生学院卫生毒理学教研室;
【基金】：国家自然科学基金(81502837) 辽宁省科技厅基金(2015020367)
【分类号】：R114

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