色氨酸对应激小鼠免疫、神经递质和行为的调节

发布时间：2018-05-11 13:19

本文选题：色氨酸 + 环磷酰胺　；参考：《江南大学》2012年硕士论文

【摘要】：本文研究了色氨酸对免疫抑制小鼠和慢性不可预见性应激小鼠氧化应激,免疫功能和行为的影响,并对相应指标做了相关性分析,通过色氨酸对小鼠氧化应激,免疫功能、单胺类神经递质含量和行为等调节作用的研究而进一步探讨色氨酸的免疫调节及抗应激作用。免疫抑制实验模型,60只昆明种雄性小鼠预饲一周后随机分为4组,每组15只:正常对照组(NC组)、环磷酰胺免疫抑制组(CTX组)、环磷酰胺-低剂量色氨酸组(CTX-L组)和环磷酰胺-高剂量色氨酸组(CTX-H组)。实验期间腹腔注射环磷酰胺,灌胃生理盐水和色氨酸。实验结束,收集血样及组织样品,中性红法测定巨噬细胞吞噬能力,酶联免疫法测定小鼠IFN-γ和皮质醇水平。慢性不可预见性应激实验模型,60只昆明种雄性小鼠预饲一周后随机分为4组,每组15只:正常对照组(NC组)、慢性不可预见性应激模型组(CUS组)、慢性不可预见性应激-低剂量色氨酸组(CUS-L组)和慢性不可预见性应激-高剂量色氨酸组(CUS-H组)。实验期间灌胃生理盐水和色氨酸,同时给予相应的应激源刺激。实验结束,收集血样及组织样品,酶联免疫法测定下丘脑中5-HT和NE含量,水迷宫法测定小鼠空间学习和记忆能力。实验结果:(1)色氨酸可降低应激小鼠体内活性氧ROS和脂质过氧化物MDA水平(P0.05),提高应激小鼠总抗氧化T-AOC能力和超氧化物歧化酶SOD酶活(P0.05)。(2)色氨酸可提高应激小鼠的脏器指数,增加腹腔巨噬细胞吞噬能力和脾淋巴细胞增殖能力,增加血清中IFN-γ和IL-2水平(P0.05),降低血清IgG和皮质醇含量(P0.05)。(3)色氨酸可缩短定位航行实验应激小鼠的逃避潜伏期(P0.05),减少空间搜索实验小鼠首次到达原平台时间且增加穿越原平台次数(P0.05)。(4)补充色氨酸可增加应激小鼠下丘脑中5-HT含量(P0.05),降低下丘脑NE含量(P0.05),补充色氨酸还可改善应激小鼠对糖水的偏爱度同时延长小鼠冰水游泳时间(P0.05)。结论:环磷酰胺应激小鼠补充色氨酸后可提高机体抗氧化能力减轻应激小鼠的氧化应激;免疫抑制小鼠补充色氨酸后可促进血清IFN-γ的生成,降低血清皮质醇的产生,还可增加小鼠腹腔巨噬细胞吞噬能力及脾淋巴细胞的增殖能力,进而有效改善由环磷酰胺应激引起的小鼠免疫系统功能的下降;慢性不可预见性应激小鼠补充色氨酸后,通过增加5-HT水平,降低NE水平而调节应激小鼠下丘脑中单胺类神经递质的含量,通过延长小鼠冰水游泳时间而提高应激小鼠的抗疲劳能力,给予应激小鼠补充色氨酸还可通过升高血清中细胞因子IL-2水平,降低血清免疫球蛋白G浓度而调节应激小鼠的免疫机能,进而对应激小鼠的免疫,空间学习记忆力及应激行为等进行调节。
[Abstract]:The effects of tryptophan on oxidative stress, immune function and behavior in immunosuppressive mice and chronic unpredictable stress mice were studied. In order to investigate the immunomodulation and anti-stress effects of tryptophan, the content and behavior of monoamine neurotransmitters were studied. Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), cyclophosphamide immunosuppressive group (CTX group), cyclophosphamide low dose tryptophan group (CTX-L group) and cyclophosphamide high dose tryptophan group (CTX-H group). During the experiment, cyclophosphamide was intraperitoneally injected with normal saline and tryptophan. At the end of the experiment, blood samples and tissue samples were collected, phagocytosis of macrophages was measured by neutral red method, and IFN- 纬 and cortisol levels in mice were measured by enzyme-linked immunosorbent assay (Elisa). Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), chronic unpredictable stress model group (CUS group), chronic unpredictable stress-low dose tryptophan group (CUS-L group) and chronic unpredictable stress-high dose tryptophan group (CUS-H group). During the experiment, saline and tryptophan were administered intragastrically and stimulated by corresponding stressors. At the end of the experiment, blood samples and tissue samples were collected. The contents of 5-HT and NE in hypothalamus were determined by enzyme-linked immunosorbent assay (Elisa), and the spatial learning and memory abilities of mice were measured by water maze method. The results showed that tryptophan could decrease the levels of reactive oxygen species (ROS) and lipid peroxide (MDA) in stress mice, and increase the total antioxidant T-AOC ability and the activity of superoxide dismutase (SOD). The phagocytosis of peritoneal macrophages and the proliferation of splenic lymphocytes were increased. Increasing the levels of IFN- 纬 and IL-2 in serum and decreasing the contents of serum IgG and cortisol (P0.05) Tryptophan could shorten the escape latency (P0.05), reduce the first time of reaching the original platform and increase the crossing time of mice in space search experiment. Supplementation of tryptophan increased 5-HT content in hypothalamus and decreased NE content in hypothalamus of stress mice. Tryptophan supplementation also improved the preference for sugar water in stress mice and prolonged swimming time of ice water in mice. Conclusion: tryptophan supplementation in cyclophosphamide stress mice can improve the antioxidant capacity and reduce oxidative stress in stressed mice, and tryptophan supplementation in immunosuppressed mice can promote the production of serum IFN- 纬 and decrease the production of serum cortisol. It also increased the phagocytosis of peritoneal macrophages and the proliferation of spleen lymphocytes, and effectively improved the decrease of immune system function induced by cyclophosphamide stress. By increasing the level of 5-HT and decreasing the level of NE, the content of monoamine neurotransmitters in hypothalamus of stress mice was regulated, and the anti-fatigue ability of stress mice was improved by prolonging the swimming time of ice water. Supplementation of tryptophan could also regulate the immune function of stressed mice by increasing the level of cytokine IL-2 in serum and decreasing the concentration of serum immunoglobulin G. Spatial learning memory and stress behavior were regulated.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

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