灵芝孢子粉对亚慢性染镉大鼠肝脏损伤的保护作用

发布时间：2018-05-12 08:17

  本文选题：灵芝孢子粉 + MDA　； 参考：《遵义医学院》2017年硕士论文

【摘要】：目的:研究灵芝孢子粉对亚慢性染镉大鼠肝脏损伤的保护作用,并探讨其可能的保护途径。方法:健康雄性SD大鼠84只,体重(90±10)g,随机分为:正常对照组(C)、单独染镉组(Cadmium,Cd)和2个给药剂量组(灵芝孢子粉0.5g/kg,1.0g/kg+Cd Cl2,即GCd L和GCdH组),每组各21只。大鼠适应性喂养7d后,每天对GCdL组和GCdH组大鼠灌相应剂量的灵芝孢子粉,C组和Cd组灌等体积蒸馏水。Cd组、GCdL组和GCd H组每隔一天腹腔注射一次氯化镉(1.0mg/kg),每周称重1次,并观察大鼠的一般情况,并在第30天、60天、90天分批处死,每组各处死7只;颈动脉取血,快速取肝脏,生理盐水冲洗,滤纸吸干多余的水分,称重,记录,-80℃保存;全自动血生化仪检测大鼠丙氨酸氨基转移酶(Alanine aminotransferase,ALT)和天门冬氨酸氨基转移酶(Aspartate transaminase,AST)的浓度;光学显微镜观察大鼠肝脏病理组织学变化;比色法检测肝脏丙二醛(Malondialdehyde,MDA)含量;酶联免疫吸附试验(ELISA)检测大鼠肝脏IL-6含量;Real time RT-PCR检测大鼠肝脏p65 m RNA、Mt-1 m RNA、Mt-2 m RNA的表达量。结果:与正常对照组比较,60d、90d的Cd组大鼠体重明显降低(P0.05),给予灵芝孢子粉干预后,大鼠体重均有不同程度增加,在90天时,GCdH组大鼠体重明显高于Cd组(P0.05);与空白组相比,大鼠染镉后肝脏脏器系数明显增大(P0.05),给药剂量组大鼠肝脏脏器系数与Cd组比较均有不同程度降低(P0.05);与空白组相比,单独染镉组ALT、AST明显升高(P0.05),而灵芝孢子粉各剂量组ALT、AST均有不同程度降低,且与单独染镉组比较差异有统计学意义(P0.05);HE染色结果显示,单独染镉组肝组织细胞间质充血严重,细胞排列杂乱,细胞界限不清晰,而给药组以上情况均有不同程度改善,并且高剂量组改善情况优于中剂量组;比色法检测MDA结果显示,经镉处理大鼠肝脏MDA含量较空白组明显增高(P0.05),给予灵芝孢子粉干预后,在60d、90d均有降低(P0.05);在30、60、90天,与空白组相比,单独染镉组大鼠肝脏细胞因子IL-6(白介素6)含量明显升高(P0.05);随着时间的延长,Cd组、GCdL组、GCdH组IL-6含量也逐渐增加(P0.05);与单独染镉组相比,给药组大鼠肝脏Mt-1 m RNA、Mt-2 m RNA的表达量明显升高(P0.05),而p65 m RNA表达明显降低(P0.05)。结论:灵芝孢子粉对亚慢性染镉大鼠肝脏损伤有保护作用,其机制可能与抗氧化应激,降低炎性细胞因子IL-6表达和诱导MT产生有关。
[Abstract]:Aim: to study the protective effect of ganoderma lucidum spores powder on liver injury in rats with subchronic cadmium exposure and explore its possible protective pathway. Methods: 84 healthy male Sprague-Dawley rats, weighing 90 卤10 g / g, were randomly divided into two groups: normal control group, single cadmium group and two dosage groups (Ganoderma lucidum spores 0.5 g / kg 路kg ~ (-1) 路kg ~ (-1) CD _ (Cl _ 2), I. e., GCd L group and GCdH group, 21 rats in each group. After 7 days of adaptive feeding, rats in GCdL group and GCdH group were given the same dose of Ganoderma lucidum spores powder group C and CD group with the same volume distilled water. CD group and GCd H group were intraperitoneally injected with 1.0 mg / kg cadmium chloride once every other day and weighed once a week. The general condition of the rats was observed, and the rats were killed in batches on the 30th day, the 60th day and the 90th day, 7 rats were killed in each group, the blood was taken from the carotid artery, the liver was taken quickly, the saline was washed, the filter paper was sucked to dry the excess water, the weight was weighed, and the records were stored at -80 鈩，

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