昆明市腹泻人群肠致病性大肠杆菌流行特征研究

发布时间：2018-05-12 21:12

本文选题：腹泻病例 + 非腹泻研究对象　；参考：《中国人兽共患病学报》2017年04期

【摘要】：目的了解云南省昆明市肠致病性大肠杆菌(enteropathogenic Escherichia coli,EPEC)的流行特征,为我国EPEC感染引起的腹泻提供科学依据。方法在云南省昆明市4家哨点医院收集腹泻病例粪便样本1 121份,非腹泻人群粪便样本319份,进行细菌培养,选择单个菌落,提取DNA,并采用荧光定量PCR检测EPEC,同时使用结构化问卷收集研究对象基本信息。结果 EPEC在腹泻病例中的检出率高于非腹泻研究对象中的检出率(5.53%,1.88%,χ~2=7.36,P0.05)。EPEC的检出率在不同年龄组腹泻病例中有统计学差异(χ~2=17.27,P0.05),其中在2—5岁腹泻病例最高(12.12%,16/132);EPEC的检出率在腹泻病例中有明显的季节分布趋势(χ~2=12.82,P0.05),其中EPEC在夏季最高(9.00%,18/200),冬季最低(3.05%,12/394)。EPEC阳性的腹泻病例水样便占61.3%(38/62),出现脱水症状占12.9%(8/62)。结论 EPEC是昆明市人群腹泻的重要细菌性病原体之一,且EPEC感染以5岁以下儿童为主,EPEC感染容易造成脱水。
[Abstract]:Objective to investigate the epidemiological characteristics of enteropathogenic Escherichia coli in Kunming, Yunnan Province, and to provide scientific evidence for diarrhea caused by EPEC infection in China. Methods 1 121 fecal samples of diarrhea cases and 319 fecal samples from non-diarrhea population were collected from 4 sentinel hospitals in Kunming, Yunnan Province. The bacteria were cultured and a single colony was selected. DNA was extracted and EPECs were detected by fluorescence quantitative PCR, and basic information was collected by structured questionnaire. Results the detection rate of EPEC in diarrhea cases was higher than that in non-diarrhea study subjects. The detection rate of EPEC was 5.53 and 1.88. There was statistical difference in the detection rate of 蠂 ~ (2 +) ~ (7.36) (P _ (0.05) P _ (0.05) among different age groups (蠂 ~ (2) ~ (2) ~ (2) ~ (27) ~ (17. 27) P _ (0.05). The highest detection rate of EPEC was 12.1216 / 13 ~ (2) in diarrhea patients aged 2 to 5 years. The seasonal distribution trend of diarrhea cases was obvious (蠂 ~ (2) 2 ~ (12) ~ (82) P 0.05, in which EPEC was the highest in summer (9.00 / 18 / 200), and the lowest in winter was 3.05 ~ (12) / 394m. EPEC positive diarrhea cases accounted for 61.3% (38 / 62) and dehydration symptoms accounted for 12.9% / 62 ~ (2)%. Conclusion EPEC is one of the most important bacterial pathogens of diarrhea in Kunming, and EPEC infection is easy to cause dehydration in children under 5 years of age.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织热带病合作中心国家级热带病国际联合研究中心;云南省疾病预防控制中心;复旦大学生命科学学院微生物学与微生物工程系;
【基金】：国家科技重大专项(No.2008ZX10004-011;No.2012ZX10004-220) 国家卫生行业科研专项(No.201502021) 上海市公共卫生第四轮三年行动计划(GWIV-29)联合资助~~
【分类号】：R516;R181.3

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