紫薯提取物对秀丽隐杆线虫抗氧化作用的影响

发布时间：2018-05-15 03:39

本文选题：秀丽隐杆线虫 + 紫薯提取物　；参考：《食品科学》2017年23期

【摘要】：目的:以秀丽隐杆线虫为对象,研究紫薯提取物(purple sweet potato extract,PSPE)抗氧化作用及其可能的作用机制。方法:将秀丽隐杆线虫饲喂于含有不同质量浓度(70、140、280μg/mL)PSPE的线虫生长培养基,研究PSPE对线虫寿命、抗氧化酶活力以及相关基因表达水平、急性氧化应激的影响。结果:PSPE能明显延长秀丽隐杆线虫的寿命,提高超氧化物歧化酶和过氧化氢酶活力,上调daf-16、ctl-1、sod-3、sir-2.1基因表达水平,下调age-1、daf-2基因表达水平,同时延长在H_2O_2、胡桃醌及百草枯氧化应激的存活时间。结论:PSPE能够有效延缓秀丽隐杆线虫的衰老、抑制脂褐素累积、提高抗氧化酶活力及抗氧化基因表达水平,增强对H_2O_2、胡桃醌及百草枯急性氧化应激的抵抗能力。
[Abstract]:Objective: to study the antioxidation effect of purple sweet potato extract PSPE and its possible mechanism. Methods: to study the effects of PSPE on nematode longevity, antioxidant enzyme activity, gene expression level and acute oxidative stress in a growth medium containing 70140280 渭 g/mL)PSPE of different concentrations of Rhizoma elegans in order to study the effects of PSPE on nematode longevity, antioxidant enzyme activity, gene expression level and acute oxidative stress. Results the life span and activity of superoxide dismutase (SOD) and catalase (catalase) were significantly prolonged, the expression of daf-16ctl-1ctl-1sod-3sir-2.1 gene was up-regulated, and the expression level of age-1 daf-2 gene was down-regulated by weight PSPE, and the activity of superoxide dismutase (SOD) and catalase (catalase) were increased. At the same time, the survival time of oxidative stress in H _ 2O _ 2, H _ 2O _ 2, H _ 2O _ 2 and paraquat was prolonged. Conclusion: PSPE can effectively delay the senescence, inhibit the accumulation of lipofuscin, increase the activity of antioxidant enzymes and the expression of antioxidant genes, and enhance the resistance to acute oxidative stress of H2O2, Nutcrone and paraquat.
【作者单位】：天津科技大学食品工程与生物技术学院;天津科技大学生物工程学院;
【基金】：国家自然科学基金应急管理项目(31540086)
【分类号】：R151

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