放射工作人员周围血GADD45 mRNA表达与受照剂量关系

发布时间：2018-05-15 11:54

本文选题：放射工作人员 + 生长抑制和DNA损伤基因　；参考：《中国职业医学》2017年03期

【摘要】：目的分析生长抑制和DNA损伤基因45(GADD45)mRNA在放射工作人员周围血中的表达情况及其与受照剂量的关系。方法采用典型抽样方法,以广东省某医院97名放射工作人员为放射组,以不接触电离辐射及其他职业性有害因素的48名医院行政工作人员为对照组,采用实时定量聚合酶链式反应法检测2组人员周围血中GADD45 mRNA表达水平。采用热释光剂量法检测和收集放射组人员过去1年的个人剂量。结果放射组人员周围血GADD45 mRNA相对表达水平高于对照组(P0.01)。放射组人员个人剂量、性别、年龄、工龄、吸烟、饮酒等因素均与GADD45 mRNA相对表达水平无相关关系(P0.05)。结论电离辐射可导致放射工作人员周围血中GADD45 mRNA表达上调,但GADD45 mRNA表达与其个人剂量无线性关系。
[Abstract]:Objective to analyze the expression of growth inhibition and DNA damage gene 45(GADD45)mRNA in peripheral blood of radiation workers and its relationship with radiation dose. Methods 97 radiation workers in a Guangdong hospital were selected as radiation group and 48 hospital administrative staff who were not exposed to ionizing radiation and other occupational harmful factors as control group. The expression of GADD45 mRNA in peripheral blood of two groups was detected by real-time quantitative polymerase chain reaction. A thermoluminescence dose method was used to detect and collect the individual doses of the radiation group in the past year. Results the relative expression of GADD45 mRNA in peripheral blood of radiation group was higher than that of control group (P 0.01). There was no correlation between individual dose, sex, age, length of service, smoking, alcohol consumption and relative expression of GADD45 mRNA in radiation group (P 0.05). Conclusion the expression of GADD45 mRNA in peripheral blood of radiation workers can be upregulated by ionizing radiation, but the expression of GADD45 mRNA is correlated with the radioactivity of individual dose.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;
【基金】：广东省医学科研基金(A2014076) 广东省职业病防治重点实验室(2012A061400007)
【分类号】：R144.1

【参考文献】