亚慢性砷暴露对小鼠精子发育及Uty基因表达影响

发布时间：2018-05-15 12:45

本文选题：三氧化二砷(AsO) + 精子发育　；参考：《中国公共卫生》2015年06期

【摘要】：目的探讨亚慢性砷暴露对小鼠精子发育和调控基因Uty表达影响。方法雄性小鼠随机分为对照组、三氧化二砷(As2O3)1、2、4 mg/L暴露组,每组10只,自然饮用含不同浓度As2O3水染毒,连续暴露60 d。取双侧睾丸、附睾,称重计算脏器系数,显微镜下观察精子活动度,苏木素-伊红染色观察精子畸形率和生精周期各级生精细胞数量,实时定量PCR检测Y染色体Uty基因表达。结果对照组、1、2、4 mg/L染砷组小鼠睾丸脏器系数分别为(3.6±0.41)、(3.47±0.41)、(3.15±0.46)、(3.14±0.45),附睾脏器系数分别为(0.82±0.14)、(0.80±0.12)、(0.70±0.11)、(0.68±0.11),与对照组比较,2、4 mg/L染砷组小鼠睾丸、附睾脏器系数明显降低(P0.05);2、4 mg/L染砷组小鼠精子活动度分别为82%、81%,明显低于对照组(97%),差异有统计学意义(P0.05),4 mg/L染砷组小鼠精子畸形率(6.6%)明显高于对照组(2.6%)(P0.05)。与对照组比较,4 mg/L染砷小鼠睾丸中A型精原细胞、前细线期精母细胞、中粗线期精母细胞、第七阶段精细胞数量[分别为(33.4±3.8)%、(4.0±4.1)%、(125±1.2)%、(220±18.8)%]明显下降(P0.05);与对照组比较,2、4 mg/L砷染毒小鼠睾丸和附睾内Uty基因表达下降,差异均有统计学意义(P0.05)。结论亚慢性砷暴露影响小鼠精子发育,其雄性生殖毒性作用可能与Uty基因表达下调有关。
[Abstract]:Objective to investigate the effects of subchronic arsenic exposure on sperm development and regulatory gene Uty expression in mice. Methods male mice were randomly divided into control group (n = 10) exposed to arsenic trioxide (as _ 2O _ 3) _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3O _ 3 (n = 10) for 60 days. Bilateral testis, epididymis, weighing organ coefficient, sperm motility were observed under microscope, sperm malformation rate and spermatogenic cell number in spermatogenic cycle were observed by hematoxylin eosin staining, and Uty gene expression on Y chromosome was detected by real-time quantitative PCR. Results the testicular organ coefficients of the control group were 3. 6 卤0. 41 卤0. 41 and 3. 15 卤0. 46 respectively, and the organ coefficients of the epididymis were 0. 82 卤0. 14 卤0. 12 卤0. 70 卤0. 11 卤0. 68 卤0. 11 respectively. Compared with the control group, the testis of the mice exposed to arsenic for 2 mg/L were compared with those of the control group. The sperm motility of mice exposed to arsenic in the epididymis group was significantly lower than that in the control group. The sperm deformity rate of the mice in the P0.05 mg/L group was significantly higher than that in the control group (2.6%), and the sperm motility of the mice in the arsenic exposed group was significantly lower than that in the control group (P 0.05%). Compared with the control group, type A spermatogonium cells, spermatocytes in the anterior fine line phase and spermatocytes in the middle thick thread stage were found in the testis of mice exposed to arsenic for 4 mg/L. In the seventh stage, the number of spermatocytes [33.4 卤3.8 + 4. 0 卤4. 1] significantly decreased P0. 05%, compared with the control group, the expression of Uty gene in testis and epididymis of mice exposed to arsenic was significantly lower than that in the control group (P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, respectively). Conclusion Subchronic arsenic exposure may affect sperm development in mice, and its male reproductive toxicity may be related to the down-regulation of Uty gene expression.
【作者单位】：大连市疾病预防控制中心;大连医科大学公共卫生学院;
【基金】：国家自然科学基金(30571584,81102160)
【分类号】：R114

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