母源性丙烯酰胺暴露对断乳期大鼠额叶皮质GAP-43和BDNF表达的影响

发布时间：2018-05-15 17:37

本文选题：丙烯酰胺 + 神经发育毒性　；参考：《神经解剖学杂志》2017年01期

【摘要】：目的:探讨母源性丙烯酰胺(acrylamide,ACR)暴露对断乳期大鼠额叶皮质生长相关蛋白(GAP-43)和脑源性神经营养因子(BDNF)表达的影响。方法:SD孕鼠随机分为4组,怀孕第6 d起对照组和实验组分别给予双蒸水和50,100,200μg/ml ACR饮水,直至仔鼠出生21 d,染毒结束时对断乳期大鼠称重。免疫组织化学检测额叶皮质GAP-43和BDNF的表达。结果:与对照组相比,实验各组断乳期大鼠体重呈浓度依赖性下降,且组间差异明显(P0.05)。与对照组相比,ACR实验各组GAP-43表达显著降低(P0.05),而低、中剂量组之间差异不显著(P0.05),高剂量组与低、中剂量组相比,表达显著减少(P0.05);与对照组相比,ACR实验组各组BDNF表达显著减少(P0.05),中、高剂量组与低剂量组相比,BDNF表达减少(P0.05),而中、高剂量组之间差异不显著(P0.05)。结论:母源性ACR染毒可能通过下调额叶皮质神经元GAP-43和BD)NF表达,抑制轴突延伸或扰乱神经元发育的内环境稳态,进而影响额叶皮质的发育。
[Abstract]:Aim: to investigate the effects of maternal acrylamide (ACR) exposure on the expression of GAP-43 in frontal cortex and BDNF in rat frontal cortex. Methods Twenty SD pregnant rats were randomly divided into 4 groups. From the 6th day after pregnancy, the control group and the experimental group were given double steamed water and 50100200 渭 g/ml ACR drinking water, respectively, until 21 days after birth, and the weaning rats were weighed at the end of the exposure. The expression of GAP-43 and BDNF in frontal cortex was detected by immunohistochemistry. Results: compared with the control group, the body weight of the weaning rats decreased in a concentration-dependent manner, and the difference between the two groups was significant (P 0.05). Compared with the control group, the expression of GAP-43 in the ACR experimental group was significantly lower than that in the control group, but in the middle dose group there was no significant difference between the two groups. The expression of BDNF in the high dose group was significantly lower than that in the low group and in the middle dose group, and the expression of BDNF in the control group was significantly lower than that in the control group, and the expression of BDNF in the experimental group was significantly lower than that in the control group. The expression of BDNF in high dose group was lower than that in low dose group, but there was no significant difference between high dose group and high dose group. Conclusion: maternal ACR exposure may inhibit the extension of axons or disturb the homeostasis of neurons by down-regulating the expression of GAP-43 and BD)NF in the frontal cortex and thus affect the development of the frontal cortex.
【作者单位】：肇庆医学高等专科学校;广东药科大学;
【基金】：广东省医学科学基金项目(A201618) 广东省中医药局中医药常规科研项目(20162156) 肇庆市科技创新指导项目(2014E184) 肇庆市医学高等专科学校青年科技基金(zqyq16-001)
【分类号】：R114

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