一起韦太夫雷登沙门菌食物中毒的分子病原学分析

发布时间：2018-05-15 23:05

本文选题：韦太夫雷登沙门菌 + 食物中毒　；参考：《现代预防医学》2017年19期

【摘要】：目的对一起韦太夫雷登沙门菌食物中毒进行分子病原学分析。方法对分离株采用肉汤稀释法进行药敏试验测定;采用PCR技术检测毒力基因inv A、sop B、flg K、ssaR和spv B的携带情况;采用ERIC-PCR技术进行基因分型检测。结果 18株韦太夫雷登沙门菌对氨苄西林、三代头孢菌素、喹诺酮类、磺胺类等抗生素均表现出敏感,临床抗生素治疗效果好;均携带染色体毒力基因inv A、sop B、flg K和ssaR,不携带质粒毒力基因spv B;ERCI-PCR显示DNA指纹图谱的相似度大于91%,可划分为1个基因型。结论该起食物中毒由同一基因型的韦太夫雷登沙门菌菌株所致,携带有多种染色体毒力基因,致病性较强,应受到高度重视。
[Abstract]:Objective to analyze the molecular etiology of a food poisoning caused by Salmonella deltaeus. Methods the isolates were tested for drug sensitivity by broth dilution method, PCR technique was used to detect the carrying of virulence genes inv Asop Bnflg KnssaR and spv B, and ERIC-PCR technique was used to detect the genotyping of the virulence genes. Results 18 strains of Wetafraden Salmonella were sensitive to ampicillin, third generation cephalosporins, quinolones, sulfonamides and so on. All of them carried chromosome virulence genes inv Asop Bnflg K and ssaR. the similarity of DNA fingerprinting showed that the similarity of DNA fingerprinting was higher than 91%, and could be divided into one genotype without plasmid virulence gene spv BnERCI-PCR. Conclusion the food poisoning was caused by the same genotype of Salmonella wetafedianensis, which carried many kinds of chromosome virulence genes and had strong pathogenicity, so it should be paid more attention to.
【作者单位】：成都大学附属医院检验科;四川省妇幼保健院检验科;成都医学院检验医学院;
【基金】：四川省卫生和计划生育委员会科研课题(编号17PJ518)
【分类号】：R155.31

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