2009-2012年安徽省人肠道病毒71型的分子流行病学特征

发布时间：2018-05-16 11:24

本文选题：肠道病毒属 + VP基因　；参考：《中华疾病控制杂志》2015年04期

【摘要】：目的探讨安徽省手足口病患儿中肠道病毒71型(human enterovirus 71,HEV71)的分子流行病学特征。方法对2009-2012年HEV71所致手足口病患儿的19份咽拭子标本进行HEV71病毒VP1区全基因核苷酸序列测定,其中1份标本来自河南信阳患儿,其余均来自安徽省辖区患儿。所得序列与国内外HEV71毒株的核苷酸序列做比对分析,构建进化树。结果 19株病毒核酸VP1区全基因均为891个核苷酸,编码297个氨基酸。所得序列与A、B、C基因型的核苷酸同源性分别为82.6%~83.6%、83.3%~85.5%、86.7%~98.9%;氨基酸同源性分别为96.0%~97.0%、96.0%~97.7%、97.0%~100.0%。结论六安市2009-2012年HEV71流行株在种系进化上与我国大陆近几年分离的毒株具有较高的同源性(94.6%~98.9%),同属于C4a基因亚型,与中国大陆前几年分离株C4b基因亚型较近。
[Abstract]:Objective to investigate the molecular epidemiological characteristics of enterovirus 71 (enterovirus 71) in children with hand, foot and mouth disease (HFMD) in Anhui province. Methods A total of 19 pharyngeal swabs from children with hand-foot-mouth disease caused by HEV71 from 2009 to 2012 were analyzed for the nucleotide sequence of the VP1 region of HEV71 virus. One of them was from Xinyang, Henan province, and the rest were from the district of Anhui province. The sequences were compared with the nucleotide sequences of HEV71 strains at home and abroad to construct an evolutionary tree. Results the nucleic acid VP1 region of 19 strains were all 891 nucleotides, encoding 297 amino acids. The nucleotide homology of the obtained sequence was 82.6 and 83.6, respectively. The nucleotide homology was 83.6 and 85.55.5and 86.7and 98.9, respectively, and the amino acid homology was 96.00.97.0and 97.797. 0 and 100.0, respectively. Conclusion the phylogeny of HEV71 epidemic strains in Luan City from 2009 to 2012 has a high homology with the strains isolated in mainland China in recent years, belonging to the subtype of C4a gene, which is close to the subtype of C4b gene isolated from the mainland of China in the past few years.
【作者单位】：六安市疾病预防控制中心;
【基金】：安徽省预防医学与公共卫生科研课题(2011Y0111)
【分类号】：R725.1;R181.3

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