鳕鱼皮胶原蛋白肽对小鼠慢性肝损伤的保护机制

发布时间：2018-05-17 08:08

本文选题：鳕鱼皮 + 胶原蛋白肽　；参考：《中国食品学报》2017年06期

【摘要】：目的:探究鳕鱼皮胶原蛋白肽对小鼠慢性肝损伤的保护作用机制,为肝病患者功能食品的开发提供理论和试验依据。方法:采用腹腔注射CCl4建立小鼠慢性肝损伤模型,检测鳕鱼皮胶原蛋白肽对小鼠血清中转氨酶和肝组织匀浆中抗氧化酶以及丙二醛的影响;HE染色观察小鼠肝组织病理学变化;蛋白印迹法测定肝组织Bax,Bcl-2,Cleavage Caspase-3,Caspase-9,Cytosolic Cyt c,TNF-α,JNK,P-JNK蛋白的表达情况。结果:与模型组比较,高剂量组(400 mg/kg)小鼠血清中AST、ALT活性、肝组织匀浆中MDA含量显著降低,而肝组织匀浆中SOD活性显著升高,且有显著的剂量关系;小鼠肝组织损伤情况得到明显改善;Bcl-2蛋白表达量增加,Bax,Cleavage Caspase-3,Caspase-9,Cytosolic Cyt c,TNF-α,P-JNK蛋白表达量降低。结论:鳕鱼皮胶原蛋白肽可增强慢性肝损伤小鼠的抗氧化活性,抑制氧化应激,对慢性肝损伤有较好的保护作用。
[Abstract]:Aim: to investigate the protective mechanism of cod skin collagen peptide on chronic liver injury in mice, and to provide theoretical and experimental basis for the development of functional food for patients with liver disease. Methods: the chronic liver injury model of mice was established by intraperitoneal injection of CCl4. The effects of cod collagen peptide on serum aminotransferase, antioxidant enzyme and malondialdehyde in liver tissue homogenate of mice were detected. The pathological changes of liver tissue were observed by HE staining. The expression of Bax-Bcl-2Cleavage Cleavage Caspase-3, cytosolic Cyt and TNF- 伪 in liver tissue was determined by Western blot. Results: compared with the model group, the alt activity in serum and the content of MDA in liver homogenate were significantly decreased in high dose group (400 mg / kg), while the activity of SOD in liver homogenate was significantly increased in a dose-dependent manner. The expression of Bcl-2 protein was significantly improved in mice liver tissue injury. The expression of Cleavage Cleavage Caspase-3, Caspase-9, cytosolic Cyt, TNF- 伪 and P-JNK protein was decreased. Conclusion: Cod skin collagen peptide can enhance the antioxidant activity of chronic liver injury mice, inhibit oxidative stress, and have a better protective effect on chronic liver injury.
【作者单位】：浙江海洋学院食品与医药学院;浙江省海洋生物医用制品工程技术研究中心;浙江省海洋水产研究所;浙江海力生集团有限公司;
【基金】：浙江省科技厅重大专项(2013C03036,2011C02003) 舟山市科技计划项目(2012C21013)
【分类号】：R151

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