香烟烟雾提取物对MIN6细胞JNK及p38MAPK表达影响

发布时间：2018-05-19 00:28

本文选题：香烟烟雾提取物(CSE) + 胰岛细胞　；参考：《中国公共卫生》2016年12期

【摘要】：目的探讨体外香烟烟雾提取物(CSE)对胰岛MIN6细胞形态及活力的影响,及对胞内丝裂原活化蛋白激酶(MAPKs)信号转导通路ERK、JNK、p38MAPK表达的影响。方法体外培养MIN6细胞至生长对数期后,设不同浓度CSE(20、50、100和200μg/m L)实验组,同时设对照组,48 h后,镜下观察MIN6细胞形态学改变,并应用CCK-8法检测CSE对MIN6细胞的增殖抑制率;同时应用蛋白印迹法(WB)检测细胞内ERK、JNK、p38M APK蛋白及磷酸化程度。结果与对照组比较,100和200μg/m L剂量下的细胞形态出现明显异常,胞核固缩,细胞呈梭形样改变;与对照组比较,MIN6细胞活力出现下降,20、50、100、200μg/m L各组细胞活力分别为(97.32±2.67)%、(94.67±5.33)%、(87.71±12.3)%和(74.23±25.8)%;与对照组相比,ERK、JNK、p38MAPK蛋白表达变化不大,但随着CSE剂量的增加,p-JNK和p-p38MAPK磷酸化程度明显增加(与对照组相比,P0.05),并呈一定的剂量-效应关系。结论 CSE能够导致M IN6细胞形态异常、抑制其细胞增殖活力,其机制可能与JNK和p38MAPK信号通路的激活相关。
[Abstract]:Objective to investigate the effects of cigarette smoke extract (CSE) on the morphology and activity of islet MIN6 cells and on the expression of ERK- JNK-p38 MAPK signal transduction pathway in vitro. Methods MIN6 cells were cultured to the logarithmic phase in vitro. The experimental groups with different concentrations of CSEX 2050100 and 200 渭 g / mL were set up. The morphological changes of MIN6 cells were observed under microscope after 48 hours in the control group, and the inhibitory rate of CSE on the proliferation of MIN6 cells was measured by CCK-8 assay. At the same time, the protein and phosphorylation of p38M APK were detected by Western blot. Results compared with the control group, the cells at the dose of 100 渭 g / mL and 200 渭 g / mL showed obvious abnormal morphology, nuclear pyknosis and spindle shape. Compared with the control group, the cell viability of the two groups were 97.32 卤2.67 卤94.67 卤5.33, 87.71 卤12.3% and 74.23 卤25.8%, respectively, and the expression of JNKp38 MAPK protein was not changed compared with the control group. However, the phosphorylation of p-JNK and p-p38MAPK increased with the increase of CSE dose (compared with the control group, P 0.05), and showed a dose-effect relationship. Conclusion CSE can induce abnormal morphology of M IN6 cells and inhibit the proliferation of M IN6 cells. The mechanism may be related to the activation of JNK and p38MAPK signaling pathways.
【作者单位】：东南大学公共卫生学院;江苏省疾病预防控制中心慢性非传染病防制所;
【基金】：国家自然科学基金(81573199) 江苏省自然科学基金(BK20151593)
【分类号】：R114

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