北京市一些地区生肉标本中单增李斯特菌的分离及其分子流行病学特征分析

发布时间：2018-05-20 00:22

本文选题：单增李斯特菌 + 血清型分型　；参考：《中国人兽共患病学报》2015年05期

【摘要】：目的了解北京市一些地区生肉中单增李斯特菌的污染情况及其分子流行病学特征。方法采用北欧食品分析标准(NMKL)对北京市一些地区采集的340份牛、羊、猪、鸡和鸭生肉标本进行单增李斯特菌的分离鉴定,并对分离菌株进行血清学分型(Serotyping)、脉冲场凝胶电泳(Pulsed Field Gel Electrophoresis,PFGE)分型及多位点序列分型(Multilocus Sequence Typing,MLST)分析。结果生肉标本中单增李斯特菌污染率为6.2%(21/340)。分离菌株含3种血清型(1/2a,1/2b和1/2c),以1/2c为优势血清型(占47.6%)。PFGE分析中,使用内切酶AscI酶切电泳将21株单增李斯特菌分为12个型别,可聚类为3个群。MLST分析将21株单增李斯特菌分为7个ST型,其中ST9型最多(10株)。结论北京市一些地区的生肉类食品中存在6.2%的单增李斯特菌污染率,这些单增李斯特菌以家系II菌株占据绝对优势(80.1%),其中1/2c型,GX6A16.CN0004和ST9型分别为血清型、PFGE带型以及MLST序列型的优势型别,与我国食源性单增李斯特菌的优势菌群特征一致。
[Abstract]:Objective to investigate the contamination and molecular epidemiological characteristics of Listeria monocytogenes in raw meat in some areas of Beijing. Methods 340 samples of beef, sheep, pig, chicken and duck raw meat collected from some areas of Beijing were isolated and identified by NMKL, a Nordic food standard, for isolation and identification of Listeria monocytogenes. Serological typing, pulsed Field Gel electrophoresis (PFGE) typing and multilocus Sequence typing (MLSTs) were performed on the isolated strains. Results the contamination rate of Listeria monocytogenes in raw meat samples was 6.2%. The isolated strains contained 3 serotypes, 1 / 2a / 2b and 1 / 2cr, with 1 / 2c as the dominant serotype (in 47.6%).PFGE analysis, 21 strains of Listeria monocytogenes were divided into 12 types by endonuclease AscI digestion electrophoresis). The 21 strains of Listeria monocytogenes were divided into 7 St types by cluster analysis of 3 groups. Among them, the ST9 type was the most 10 strains. Conclusion the contamination rate of Listeria monocytogenes is 6.2% in raw meat food in some areas of Beijing. These strains of Listeria monocytogenes dominated 80.1% among them, 1 / 2c type GX6A16.CN0004 and ST9 type were serotype PFGE band type and MLST sequence type, respectively, which were consistent with the dominant flora characteristics of Foodborne Listeria monocytogenes (Listeria monocytogenes) in China.
【作者单位】：传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所;贵阳医学院微生物学教研室;郑州市第六人民医院皮肤性病科;
【基金】：国家重大传染病防治科技重大专项(No.2011ZX10004-001,No.2013ZX10004-101)联合资助~~
【分类号】：R155.5

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