敌敌畏对肾细胞系293T增殖的影响

发布时间：2018-05-24 23:33

本文选题：肾细胞T + 敌敌畏　；参考：《基因组学与应用生物学》2017年03期

【摘要】：为了研究敌敌畏(dichlorvos,DDVP)对人肾细胞的毒性效应。应用MTT比色法检测敌敌畏对人肾细胞系293T细胞增殖的影响,流式细胞仪检测293T细胞的凋亡情况,荧光二氯荧光素二乙酸(DCF-DA)测定细胞内的活性氧(reactive oxidative species,ROS)水平。结果表明:用浓度为0.5 mmol/L、1 mmol/L、5 mmol/L、10 mmol/L、15 mmol/L和20 mmol/L的敌敌畏分别处理293T细胞1 h后发现敌敌畏对293T的生长抑制具有剂量效应,计算得到1 h的IC_(50)值为10.406 mmol/L;不同浓度敌敌畏(0.5 mmol/L,5 mmol/L,10 mmol/L)处理1 h 293T细胞发现细胞产生明显的凋亡现象,随着处理浓度的增加,细胞的凋亡率升高,检测细胞内活性氧水平发现细胞内活性氧水平也随之升高。结果提示敌敌畏能够通过凋亡途径抑制293T细胞的增殖,其作用机制可能是通过增加293T细胞内活性氧水平,促进细胞凋亡,进而抑制细胞增殖。
[Abstract]:To study the toxic effect of dichlorvosine DDVP on human renal cells. The effects of dichlorvos on the proliferation of human renal cell line 293T were detected by MTT colorimetry, apoptosis of 293T cells was detected by flow cytometry, and reactive oxidative speciesros were measured by fluorescence dichlorofluorescein diacetate (DCF-DAA). The results showed that dichlorvos at the concentration of 0.5 mmol / L ~ (-1) 5 mmol / L ~ (10) mmol 路L ~ (-1) and 10 mmol / L ~ (15) mmol/L and 20 mmol/L, respectively, were used to treat 293T cells for 1 h, and the results showed that dichlorvos had a dose effect on the growth inhibition of 293T cells. After 1 h treatment with 0.5 mmol / L dichlorvos (0.5 mmol / L) and 10 mmol / L (10 mmol / L) of dichlorvos, it was found that the apoptosis of 293T cells was obvious with the increase of the concentration of dichlorvos, and the apoptosis rate of the cells increased with the increase of the concentration of dichlorvos. Detection of intracellular reactive oxygen species showed that intracellular reactive oxygen species levels also increased. The results suggested that dichlorvos could inhibit the proliferation of 293T cells through the apoptotic pathway, and its mechanism might be to promote cell apoptosis by increasing the level of reactive oxygen species in 293T cells and thus inhibit cell proliferation.
【作者单位】：贵州大学农业生物工程重点实验室/生命科学学院;贵州大学动物科学学院;
【基金】：国家自然科学基金(No.31401091) 贵州省科学技术基金(黔科合J字[2013]2100号) 贵州大学研究生创新基金(No.研农2016002)共同资助
【分类号】：R114

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